# Bioremediation of lunar regolith simulant through mycorrhizal fungi and plant symbioses enables chickpea to seed

## TL;DR

Researchers tested using fungi and compost to grow chickpeas in moon-like soil, showing plants can survive and even thrive with the help of these biological methods.

## Contribution

This study demonstrates the feasibility of using mycorrhizal fungi and vermicompost to grow chickpeas in lunar regolith simulant, enabling future space agriculture.

## Key findings

- Chickpeas successfully seeded in mixtures with up to 75% lunar regolith simulant when inoculated with AMF.
- AMF inoculation extended plant survival in 100% lunar regolith simulant by two weeks compared to non-inoculated plants.
- AMF colonized roots in all mixtures, including 100% lunar regolith simulant, showing symbiosis under extreme conditions.

## Abstract

Food sustainability is a significant challenge for long-term space travel. Plants can provide fresh nutrition, reducing reliance on packaged foods. Using Lunar regolith simulant (LRS), we tested a methodology to create a productive growth medium for horticultural crops on the Moon. We leveraged chickpea (Cicer arietinum), Arbuscular Mycorrhizal Fungi (AMF), and Vermicompost (VC) to enhance plant stress tolerance, sequester contaminants, and improve substrate structure. Chickpeas were cultivated in LRS/VC mixtures, with or without AMF, under climate-controlled conditions. Plants seeded successfully in mixtures containing up to 75% LRS when inoculated with AMF. While the number of seeds declined with increasing LRS concentration, seed size remained stable. Higher LRS concentrations induced stress; however, plants grown in 100% LRS inoculated with AMF demonstrated an average extension of two weeks in survival compared to non-inoculated plants. AMF colonized roots across all mixtures, including 100% LRS, demonstrating the ability to establish symbioses under extreme conditions. We also observed improvement in the structural properties of LRS by forming aggregates capable of withstanding extreme conditions, potentially mitigating particle-related hazards. These results provide a baseline for chickpea establishment and yield in amended LRS while demonstrating biological improvements in regolith properties.