# A case–control neuroimaging investigation of chronic Zika virus-infected adults

## TL;DR

This study investigates the long-term effects of Zika virus on the adult brain and finds no significant changes in brain structure or function.

## Contribution

The study provides the first detailed clinical characterization of adults with chronic Zika virus infection.

## Key findings

- Chronic Zika virus infection in adults is not associated with large brain changes.
- Multimodal analyses yielded null results in structural and functional brain measures.
- The study highlights the need for longitudinal care for adult survivors of severe Zika infections.

## Abstract

Systemic viral infections with neurotropic potential pose significant global health challenges. The Zika virus (ZIKV) is known for its pronounced neurotropism, with recent infectious clusters raising renewed public health concerns. While research has predominantly focused on congenital populations, growing evidence suggests that the mature central nervous system (CNS) is also vulnerable. However, no study has examined the long-term impact of ZIKV infection on the adult human brain.

To address this gap, we studied a rare group of adult ZIKV patients presenting with both peripheral (Guillain-Barré Syndrome; GBS) and CNS-related neurological symptoms. We compared these patients at the chronic stage (5 to 12 months post-infection) to healthy controls and to patients with GBS of non-ZIKV etiology (total N = 43). Structural and functional measures included cortical thickness, white matter hyperintensities, diffusion metrics, and resting-state functional connectivity.

Despite the rarity of both patient populations, power analyses indicated that our sample size could detect large group differences—effect sizes deemed reasonable given the severity of neurological symptoms in the ZIKV group. Nonetheless, our multimodal analyses yielded null results, with Bayesian statistics (where applicable) providing evidence for a lack of effects.

The null findings suggest that chronic ZIKV infection in adults is not associated with brain changes of large magnitude. Importantly, this study offers detailed clinical characterization of a heavily understudied group. In light of recent ZIKV outbreaks, this characterization underscores the need to monitor, study, and provide longitudinal care to adult survivors of severe ZIKV infections.