# A neuregulin-like ligand and EGF receptor underpin Echinococcus multilocularis development

## TL;DR

A neuregulin-like ligand and EGF receptor are crucial for the development of Echinococcus multilocularis, a parasite causing a severe zoonotic disease.

## Contribution

The discovery of a neuregulin-like ligand (EmNRG) and its receptor (EmER1) as key regulators of parasite stem cell division in Echinococcus multilocularis.

## Key findings

- EmER1 is essential for metacestode vesicle formation from germinative cells and is inhibited by afatinib.
- EmNRG, a neuregulin-like ligand, interacts with EmER1 and is upregulated during stem cell clonal expansion.
- Knockdown of EmNRG encoding gene impairs metacestode vesicle generation by germinative cells.

## Abstract

Alveolar echinococcosis (AE), a neglected zoonotic disease, is caused by the infiltrative growth of the metacestode larval stage of the cestode Echinococcus multilocularis within host organs. We previously showed that metacestode development relies on the mitotic activity of a population of parasite stem cells known as germinative cells. However, the molecular mechanisms that control Echinococcus stem cell dynamics such as cell-cycle progression, self-renewal, and differentiation remain poorly understood. Building on earlier reports implicating epidermal growth factor (EGF) signalling in stem cell regulation, we here characterize the parasite’s repertoire of three EGF receptors. Using RNAi and inhibitor assays, we identify one receptor, EmER1, as essential for the formation of metacestode vesicles from germinative cells. We further demonstrate that EmER1 is targeted by afatinib, an EGF receptor inhibitor with potent anti-parasitic activity both in vitro and in vivo. Through bioinformatic analyses and membrane-bound yeast two-hybrid assays, we identified a parasite-derived, neuregulin-like ligand for EmER1, termed EmNRG, whose expression is strongly upregulated in metacestode vesicles during clonal expansion of germinative cells. RNAi-mediated knockdown of the EmNRG encoding gene severely impaired the capacity of germinative cells to generate metacestode vesicles. Our data support that EmNRG and EmER1 constitute a cognate ligand - receptor system regulating the balance between asymmetric and symmetric stem cell division in E. multilocularis. These findings provide new insights into Echinococcus stem cell biology and highlight EGF signalling as a promising avenue for developing novel anti-echinococcosis therapeutics.