Substrate secretion by different EHEC secretion systems during their interaction with epithelial cells
Landy Zambrano-Arguello, Jaime Vazquez-Lopez, Fernando Navarro-Garcia
TL;DR
The study explores how different secretion systems in EHEC bacteria interact with epithelial cells during infection and influence each other's protein secretion.
Contribution
The study reveals a hierarchical and interdependent secretion pattern among EHEC secretion systems during epithelial cell interaction.
Findings
Epithelial cells enhance EHEC protein secretion through multiple secretion systems.
Mutating one secretion system affects the secretion of others, indicating interdependence.
Bioinformatics identified new potential substrates for each secretion system.
Abstract
Enterohemorrhagic Escherichia coli (EHEC) causes severe foodborne illness in humans. EHEC harbors five types of secretion systems (SSs) and unlike the type three secretion system (T3SS), the other SSs are less explored. Some substrates secreted by these SSs have been described; however, how these SSs collectively participate in EHEC-epithelial cell interaction during infection remains unknown. Here, we optimized protein secretion by four of the EHEC SSs in the absence and presence of epithelial cells, since the T6SS is not expressed in vitro. The secretion of substrates through the EHEC SSs followed a hierarchical pattern when bacteria encountered epithelial cells. Epithelial cells increase the protein secretion by EHEC and mutants in SSs (Western-blot and proteomics). Remarkably, mutants in one particular SS affect protein secretion by other SSs. Analysis of the EHEC secretome in the…
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Taxonomy
TopicsEscherichia coli research studies · Vibrio bacteria research studies · Bacillus and Francisella bacterial research