# The effect of aerobic exercise with blood flow restriction on substrate utilization and energy expenditure

**Authors:** Jakob D. Lauver, Nathen Andrews, Chase Harris, Nelo E. Zanchi, Kelly E. Johnson, Timothy R. Rotarius, Justin P. Guilkey

PMC · DOI: 10.3389/fspor.2026.1738777 · 2026-02-20

## TL;DR

This study shows that adding blood flow restriction to low-intensity aerobic exercise increases fat burning and energy expenditure more than high-intensity or regular low-intensity exercise.

## Contribution

The study introduces a new method of enhancing fat oxidation and energy expenditure through low-intensity exercise with blood flow restriction.

## Key findings

- LI-BFR increased fat oxidation more than HI and LI at 120 minutes post-exercise.
- Total excess energy expenditure was significantly higher in LI-BFR compared to HI and LI.
- The results suggest LI-BFR could be more effective for long-term weight management.

## Abstract

This study examined the effects of intermittent blood flow restriction (LI-BFR) compared to low- (LI) and high-intensity interval exercise (HI) on substrate utilization and energy expenditure (EE).

Exercise protocols consisted of 2-minute work intervals interspersed with 1-minute recovery intervals (20W), HI consisted of 5 intervals performed at a workload corresponding to 140% of ventilatory threshold intensity and LI and LI-BFR consisted of 10 intervals performed at 70% of ventilatory threshold. BFR cuffs were inflated to 80% of limb occlusion pressure during each work interval and deflated during each recovery. Following each exercise protocol subjects rested for three hours, during which excess EE and substrate utilization were measured. For all comparisons, statistical significance was set at p ≤ 0.05.

Fat oxidation rate was significantly greater in LI-BFR (0.11 ± 0.03 g min−1) compared to HI (0.09 ± 0.03 g min−1) at 120 MIN (d = 1.13). Fat oxidation in LI-BFR (60 MIN = 0.14 ± 0.01 g·min−1, 120 MIN = 0.11 ± 0.01 g min−1) was greater than LI (60 MIN = 0.08 ± 0.02 g min−1, 120 MIN = 0.9 ± 0.02 g min−1). Total excess EE was greater in LI-BFR (184.0 ± 37.6 kcals) compared to HI (127.0 ± 12.0 kcals; d = 3.23) and LI (144.83 ± 35.5 kcals; d = 2.21).

This study suggests that the addition of BFR to low-intensity results in increased fat oxidation following exercise as well as increased EE. The observed increased fat oxidation and EE could potentially have significant long-term effects on weight management.

## Full-text entities

- **Genes:** INS (insulin) [NCBI Gene 3630] {aka IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10}, SLC2A4 (solute carrier family 2 member 4) [NCBI Gene 6517] {aka GLUT4}, LIPE (lipase E, hormone sensitive type) [NCBI Gene 3991] {aka AOMS4, FPLD6, HSL, LHS, REH}, GH1 (growth hormone 1) [NCBI Gene 2688] {aka GH, GH-N, GHB5, GHN, IGHD1A, IGHD1B}
- **Diseases:** cardiovascular disease (MESH:D002318), weight loss (MESH:D015431), orthopedic-related injury (MESH:D009140), VT (MESH:D012131), hypoxic (MESH:D002534), fatigue (MESH:D005221), Excess energy expenditure (MESH:D011502), Hypoxia (MESH:D000860)
- **Chemicals:** BFR (-), Fat (MESH:D005223), caffeine (MESH:D002110), O2 (MESH:D010100), catecholamine (MESH:D002395), lactate (MESH:D019344), Carbohydrate (MESH:D002241), Bicarbonate (MESH:D001639), creatine phosphate (MESH:D010725), ATP (MESH:D000255), water (MESH:D014867), CO2 (MESH:D002245), lipid (MESH:D008055), glycogen (MESH:D006003), free fatty acid (MESH:D005230), norepinephrine (MESH:D009638), alcohol (MESH:D000438), glucose (MESH:D005947), PO2 (MESH:C093415)
- **Species:** Homo sapiens (human, species) [taxon 9606], Nicotiana tabacum (American tobacco, species) [taxon 4097]

## Figures

4 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12962658/full.md

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Source: https://tomesphere.com/paper/PMC12962658