Pseudomonas aeruginosa gene expression changes during established biofilm infection in a cystic fibrosis lung model
Niamh E. Harrington, Freya Allen, Ramón Garcia Maset, Freya Harrison
TL;DR
This study examines how Pseudomonas aeruginosa changes its gene activity during a 7-day biofilm infection in a model of the cystic fibrosis lung.
Contribution
The study provides new insights into gene expression changes in P. aeruginosa during established chronic biofilm infection in a clinically relevant model.
Findings
The EVPL model supports P. aeruginosa biofilm growth and increased antibiotic tolerance for at least 7 days.
Significant upregulation of sulfur metabolism genes was observed in the biofilm.
Antimicrobial resistance gene expression did not change significantly over time.
Abstract
The opportunistic pathogen Pseudomonas aeruginosa forms biofilm infections in the lungs of people with the genetic condition cystic fibrosis (CF) that can persist for decades. There are numerous P. aeruginosa lifestyle changes associated with chronic biofilm infection that are cued by the CF lung environment. These include a loss of virulence, metabolic changes and increased antimicrobial tolerance. We have investigated P. aeruginosa PA14 biofilm infection over 7 days in an ex vivo pig lung (EVPL) model for CF, previously shown to facilitate formation of a clinically relevant P. aeruginosa biofilm structure with expression of key genes comparable to human infection. We have compared P. aeruginosa gene expression between sequential time points: 24 h, 48 h and 7 days post-infection, and investigated tolerance to polymyxins. Our results demonstrate that the EVPL model can maintain a P.…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
Figure 15
Figure 16
Figure 17Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsBacterial biofilms and quorum sensing · Cystic Fibrosis Research Advances · Antibiotic Resistance in Bacteria