# Machine learning-based evaluation of seed priming and biostimulant applications in rainfed wheat

## TL;DR

This study explores how seed priming with zinc and foliar biostimulants can improve rainfed wheat growth and yield, using machine learning to predict outcomes.

## Contribution

The novel use of machine learning models to evaluate the combined effects of seed priming and biostimulant applications on rainfed wheat agronomic traits.

## Key findings

- ZnSO4 seed priming significantly improved spike emergence, plant height, and biological yield.
- Nano-formulated foliar biostimulants maximized grain yield and protein content when combined with ZnSO4 priming.
- Regularized regression models predicted yield traits more accurately than XGBoost/CatBoost.

## Abstract

Rainfed wheat suffers from water scarcity and micronutrient deficits, calling for innovative practices. This study tests zinc sulphate (ZnSO4) seed priming combined with foliar iron (Fe), zinc (ZN), and manganese (Mn) (various formulations) and uses multiple machine-learning models to predict agronomic outcomes.

A field experiment was conducted in northwestern Iran using a factorial randomized complete block design with four replications. Treatments included three ZnSO4 priming concentrations (0.1%, 0.2%, 0.3%) and five foliar sprays (conventional, magnetized, and nano-formulations of Fe, Zn, and Mn), plus water controls. Agronomic traits (e.g., spike emergence, plant height, yield, protein content) were measured. Data were analyzed with ANOVA and modelled using eight regression algorithms (Linear, Ridge, Lasso, Elastic Net, support vector regression (SVR), Random Forest, eXtreme Gradient Boosting (XGBoost), CatBoost).

The results demonstrated that seed priming with ZnSO4 considerably expedited spike emergence, enhanced plant height, and increased biological yield, with elevated ZnSO4 concentrations intensifying these effects. Foliar application of biostimulants enhanced yield components, grain yield, and protein-related characteristics. The maximum grain production (1,648 kg ha−1) was attained with 0.3% ZnSO4 seed priming in conjunction with nano foliar application of Fe, Zn, and Mn, indicating synergistic nutrient uptake/use or, alternatively, the potential of prediction approaches such as regularized regressions (Ridge, Lasso, Elastic Net) were most accurate for biological yield, protein content, and harvest index, whereas XGBoost/CatBoost captured nonlinearities but were less consistent for seed-related features. Overall, ZnSO4 priming combined with nano biostimulants markedly enhances rainfed wheat performance.