# Anti-Inflammatory Effect of Aniba rosaeodora Essential Oil-Loaded Chitosan Membrane

## TL;DR

This study shows that chitosan membranes infused with Aniba rosaeodora essential oil have anti-inflammatory properties and could be used as wound dressings.

## Contribution

The novel contribution is the development of chitosan membranes with anti-inflammatory effects by incorporating Aniba rosaeodora essential oil.

## Key findings

- ArEO incorporation increased thermal stability and prevented essential oil degradation.
- Membranes with ArEO significantly reduced nitrite and TNF-α levels in inflammation models.
- ArEO-loaded membranes maintained mechanical properties suitable for wound dressing applications.

## Abstract

Bioactive chitosan
(CH) membranes containing 0.5, 2.5, and 5.0%
w/w of Aniba rosaeodora essential oil
(ArEO), rich in linalool (82.9%) and cis-linalool oxide (2.9%) were
developed using the casting method. Fourier transform infrared spectroscopy
(FTIR) results demonstrated that CH chains interacted with ArEO, leading
to increased thermal stability and preventing isolated ArEO degradation.
ArEO concentrations did not influence membrane morphology. Incorporating
ArEO into the formulated membranes did not significantly alter their
Young’s modulus (41.84–48.00 MPa), which remains within
the skin’s modulus of elasticity range (0.02 to 57 MPa). They
also exhibited mucoadhesion for tissue adhesion, with no significant
difference with adding ArEO (0.135–0.161 N). Additionally,
ArEO incorporated into the developed wound dressings significantly
reduced nitrite levels in the in vitro inflammation model. Tumor necrosis
factor-alpha (TNF-α) levels in lipopolysaccharide (LPS)-stimulated
macrophages were significantly reduced at all ArEO concentrations
in the wound dressings. The developed membranes may represent a promising
alternative for wound dressings with anti-inflammatory properties.