# Development of Scaffold Based on Buriti Oil and PEG-400 at Low Temperature

## TL;DR

This study develops a biocompatible scaffold using buriti oil and PEG-400 for bone regeneration, showing promising structural and biological properties.

## Contribution

The novel use of buriti oil-based monoacylglyceride with PEG-400 to create sustainable, porous scaffolds for tissue engineering.

## Key findings

- Scaffolds Sc1 and Sc2 showed average pore sizes of 248 and 258 μm, ideal for bone regeneration.
- Cell viability was comparable to the control, indicating biocompatibility.
- Scaffolds exhibited thermal stability exceeding 200 °C and contained β-carotene.

## Abstract

Bone mass loss due to trauma or disease is a growing
problem, requiring
biocompatible and structurally stable materials for bone regeneration.
In this study, interconnected porous scaffolds were developed from
monoacylglyceride (MAG) derived from buriti oil (OB), combined with
PEG-400, to examine the feasibility of MAG/PEG-400 as a biomedical
scaffold to overcome the disadvantages of traditional implants. Fourier
transform infrared (FTIR) analyses confirmed the formation of the
scaffolds (Sc1 and Sc2), and scanning electron microscopy (SEM) images
revealed porous structures with aver-age pore sizes of 248 and 258
μm, ideal for bone regeneration. The presence of β-carotene
in the scaffolds was evidenced by UV–vis, and their thermal
stability exceeded 200 °C. MTT assays indicated cell viability
comparable to the control, while hemolysis tests revealed higher hemolytic
activity in Sc1, possibly due to PEG-400. These OB-based polyurethanes
stand out as a promising innovation, providing a suitable cellular
microenvironment for bone regeneration and representing a significant
contribution to sustainable biomaterials in tissue engineering (TE).