# The genome sequence of the Clay Triple-lines, Cyclophora linearia (Hübner, 1799) (Lepidoptera: Geometridae)

**Authors:** Laura Sivess, Gavin R. Broad, Stephanie Holt, Liam M. Crowley, Annabel Whibley, Mohadeseh Tahami

PMC · DOI: 10.12688/wellcomeopenres.25389.1 · 2025-12-19

## TL;DR

This paper presents the genome sequence of the Clay Triple-lines moth, including two haplotypes and gene annotations, as part of a larger project to sequence species in Britain and Ireland.

## Contribution

The first reference genome for Cyclophora linearia, including chromosomal pseudomolecules and mitochondrial DNA.

## Key findings

- The genome assembly includes two haplotypes totaling 280.22 and 284.23 megabases.
- Haplotype 1 is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome.
- Gene annotation identified 11,742 protein-coding genes.

## Abstract

We present a genome assembly from an individual male
Cyclophora linearia (Clay Triple-lines; Arthropoda; Insecta; Lepidoptera; Geometridae). The assembly contains two haplotypes with total lengths of 280.22 megabases and 284.23 megabases. Most of haplotype 1 (99.9%) is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 16.54 kilobases. Gene annotation of this assembly on Ensembl identified 11 742 protein-coding genes. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

## Linked entities

- **Species:** Cyclophora linearia (taxon 934830)

## Full-text entities

- **Chemicals:** Arima (MESH:D020912), AMPure (-), Poly(A) (MESH:D011061), formaldehyde (MESH:D005557), PB (MESH:D007854), TC (MESH:D013667), ethanol (MESH:D000431), Nucleotide (MESH:D009711)
- **Species:** Cyclophora linearia (species) [taxon 934830], Pterygota (genus) [taxon 300552], Quercus robur (English oak, species) [taxon 38942], Cyclophora punctaria (maiden's blush, species) [taxon 310442], Meleagris gallopavo (common turkey, species) [taxon 9103]

## Figures

6 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12961284/full.md

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Source: https://tomesphere.com/paper/PMC12961284