# Institut Georges Lopez-2M as a Novel Lung Preservation Solution Attenuates Ischemia-Reperfusion Injury in a Rat Ex Vivo Lung Perfusion Model

## TL;DR

A new lung preservation solution, IGL-2M, reduces lung injury during cold storage in a rat model, showing better protection than existing solutions.

## Contribution

IGL-2M is a novel preservation solution that effectively reduces ischemia-reperfusion injury in lungs.

## Key findings

- IGL-2M reduced edema formation and improved lung compliance compared to OCS.
- IGL-2M showed lower inflammatory markers in perfusate and bronchoalveolar lavage.
- IGL-2M was not inferior to Perfadex Plus and reduced TNF-α expression.

## Abstract

Institut
Georges Lopez-2M (IGL-2M), a novel preservation solution containing polyethylene glycol (PEG 35kD, 5 g/L), preserves mitochondrial integrity and redox balance in liver grafts. This study assesses IGL-2M’s effect on lung preservation during prolonged cold ischemia. Rat’s heart-lung blocks were procured and subjected to 18 h cold ischemia (4 °C). Lungs were flushed and preserved using one of these preservation solutions: OCS, Perfadex Plus, IGL-2M (n = 6/group). Following ischemia, lungs underwent up to 7 h normothermic ex vivo lung perfusion. Edema was quantified by weight gain. Lung physiological parameters were recorded. Perfusate, bronchoalveolar lavage (BAL), and tissue samples were collected. All lungs in IGL-2M group completed 7 h EVLP protocol. Compared to OCS, IGL-2M reduced edema formation (p < 0.01), preserved superior compliance (p < 0.01), and maintained lower pulmonary vascular resistance (p < 0.01). IGL-2M showed lower perfusate concentrations of IL-1β (p < 0.05), IL-6 (p < 0.05), and TNF-α (p = 0.08). In BAL, IGL-2M reduced IL-1β (p < 0.01), IL-6 (p < 0.05), TNF-α (p < 0.01), and CXCL1 (p < 0.01). IGL-2M showed lower release of Syndecan-1 (p < 0.05). Compared to Perfadex Plus, IGL-2M was not inferior, with reduced expression of TNF-α in the perfusate (p < 0.05). IGL-2M effectively prevents edema development like Perfadex Plus. IGL-2M results in decreased inflammation and a stronger endothelial lining, making it a promising solution for lung preservation.

Scientific summary graphic comparing OCS, Perfadex Plus, and IGL-2M preservation solutions in a rat lung perfusion model following cold ischemia, featuring schematic, photo, and two data plots showing IGL-2M reduces edema and strengthens vascular integrity relative to controls.