# Field-based hyperspectral characterization of wetland plant diversity and vitality in Burullus Lagoon (Nile Delta, Egypt)

**Authors:** Ghada A. Khdery, Mohamed S. Shokr, Nazih Y. Rebouh, Yasser A. El-Amier

PMC · DOI: 10.1371/journal.pone.0341891 · PLOS One · 2026-03-04

## TL;DR

This study uses hyperspectral data to characterize plant diversity and health in Burullus Lagoon, creating a reference library for biodiversity monitoring.

## Contribution

The study provides a curated field-based hyperspectral library for wetland species in Burullus Lagoon, supporting biodiversity and environmental monitoring.

## Key findings

- Red and near-infrared spectral regions best distinguish wetland plant species.
- Dominant species like Phragmites australis show distinct near-infrared reflectance due to canopy and biochemical differences.
- Most species show healthy vitality, but interspecific variability indicates differing stress responses.

## Abstract

Burullus Lagoon, situated in the Nile Delta of Egypt, is a Ramsar-listed wetland of high ecological importance, particularly in relation to its floristic diversity. This study presents a field-based hyperspectral characterization of wetland vegetation with the objective of establishing a reference spectral library to support biodiversity assessment and environmental monitoring. Hyperspectral reflectance measurements were obtained for 41 plant species selected from a total of 63 floristically identified taxa, based on ecological dominance, spatial recurrence across sampling sites (≥3 stands), and suitability for reliable field spectral acquisition. Spectroscopic data were collected from 44 stands representing lagoon shores, islets, and open-water habitats using an ASD FieldSpec spectroradiometer covering the 350–2500 nm spectral range. A set of vegetation indices was applied to evaluate key biophysical and biochemical properties associated with plant vitality, water status, and biomass. The results indicate that the red and near-infrared regions provide the highest discriminatory capability among species, whereas the shortwave infrared region exhibits more limited discriminatory capability. Dominant taxa, including Phragmites australis and Atriplex halimus, displayed elevated near-infrared reflectance, consistent with differences in canopy structure and biochemical composition. Most species showed vegetation index responses broadly indicative of healthy physiological conditions, although interspecific variability suggests contrasting stress responses among taxa. Overall, the study demonstrates the applicability of field-based hyperspectral data for species-level discrimination in wetland environments and delivers a curated spectral library to support biodiversity conservation and long-term ecosystem management at Burullus Lagoon.

## Linked entities

- **Species:** Phragmites australis (taxon 29695), Atriplex halimus (taxon 240028)

## Full-text entities

- **Diseases:** drought (MESH:C536747)
- **Chemicals:** Chlorophyll (MESH:D002734), salt (MESH:D012492), water (MESH:D014867), Carotenoid (MESH:D002338), Chlorophyll a &amp; b (-), Anthocyanin (MESH:D000872)
- **Species:** Arthrocaulon macrostachyum (species) [taxon 239181], Typha domingensis (totora, species) [taxon 189386], Potamogeton crispus (curly-leaf pondweed, species) [taxon 55318], Ceratophyllum demersum (hornwort, species) [taxon 4428], Cynanchum acutum (species) [taxon 185024], Suaeda pruinosa (species) [taxon 1494406], Cyperus alopecuroides (species) [taxon 1234173], Echinochloa stagnina (burgu grass, species) [taxon 338578], Suaeda (sea-blites, genus) [taxon 46108], Symphyotrichum subulatum var. squamatum (varietas) [taxon 1053329], Solanum nigrum (black nightshade, species) [taxon 4112], Stuckenia pectinata (sago-pondweed, species) [taxon 55444], Atriplex halimus (Mediterranean saltbush, species) [taxon 240028], Suaeda monoica (species) [taxon 224178], Malva parviflora (species) [taxon 145753], Mesembryanthemum crystallinum (common iceplant, species) [taxon 3544], Spergularia marina (lesser sea-spurrey, species) [taxon 191669], Homo sapiens (human, species) [taxon 9606], Atriplex prostrata (halberd-leaf orache, species) [taxon 190330], Tetraena alba (species) [taxon 90538], Juncus acutus (species) [taxon 308385], Atriplex portulacoides (species) [taxon 376799], Limbarda crithmoides (species) [taxon 119182], Chenopodiastrum murale (nettle-leaf goosefoot, species) [taxon 46091], Cakile maritima (European sea-rocket, species) [taxon 220483], Lemna gibba (swollen duckweed, species) [taxon 4470], Juncus rigidus (species) [taxon 1423389], Tamarix nilotica (species) [taxon 189799], Pontederia crassipes (water hyacinth, species) [taxon 44947], Alternanthera sessilis (species) [taxon 221762], Saccharum spontaneum (fodder cane, species) [taxon 62335], Mesembryanthemum nodiflorum (species) [taxon 359585], Phragmites australis (common reed, species) [taxon 29695]

## Full text

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## Figures

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## References

77 references — full list in the complete paper: https://tomesphere.com/paper/PMC12959720/full.md

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Source: https://tomesphere.com/paper/PMC12959720