# Multi-isotope analysis of mammal bones provides environmental context for the adoption of agriculture in the Tehuacan Valley of Mexico

**Authors:** Andrew D. Somerville, Isabel Casar, Rocío Hernández-Flores, Francisco Otero, Edith Cienfuegos Alvarado, Daniel Dalmas, Joaquín Arroyo-Cabrales, Kent V. Flannery, Pedro Morales-Puente, Laura E. Beramendi-Orosco

PMC · DOI: 10.1126/sciadv.adw9222 · Science Advances · 2026-03-04

## TL;DR

This study uses isotope analysis of animal bones to understand the environmental conditions during the start of agriculture in Mexico's Tehuacan Valley.

## Contribution

The paper provides new environmental context for the adoption of maize agriculture in Mesoamerica using multi-isotope analysis of mammal bones.

## Key findings

- Maize cultivation began during a relatively wet period in the Tehuacan Valley.
- Agricultural intensification and social complexity emerged during a possible dry/wet climate transition.
- Isotope data from deer and rabbit bones reflect landscape, precipitation, and temperature changes.

## Abstract

The domestication and global spread of maize (Zea mays) are pivotal processes in world history. Yet, despite the current importance of maize in global nutrition, food security, and trade, much of its origins remain debated. This paper addresses environmental explanations for the initial intensification of maize agriculture by reconstructing the paleoenvironment of the Tehuacan Valley, Puebla, Mexico. Multi-isotope analysis (δ13Cap, δ18Oap, δ13Ccol, and δ15Ncol) of deer (Odocoileus virginianus) and rabbit (Sylvilagus spp.) bones from archaeological deposits produces proxy environmental data relevant to the botanical composition of the landscape, precipitation, and temperature. Results indicate that maize cultivation began in the Tehuacan Valley during a relatively wet period, while agricultural intensification and social complexity emerged several centuries later, during a possible dry/wet transition. Stable isotope results are contextualized within broader paleoclimate and archaeological records. This study enhances our understanding of the environmental setting in which agricultural intensification first occurred in Mesoamerica and contributes to discussions on the origins of farming more broadly.

Isotopic values of animal bones reflect environmental conditions during the origins of agriculture in south-central Mexico.

## Linked entities

- **Species:** Odocoileus virginianus (taxon 9874), Zea mays (taxon 4577)

## Full-text entities

- **Genes:** td1 [NCBI Gene 103626458], LOC541854 (waxy 1) [NCBI Gene 541854] {aka 9C20.5, GBSS1, GRMZM2G024993, GbssI, Wx, mwx}, tb1 [NCBI Gene 542361], BA1 (barren stalk1) [NCBI Gene 542186] {aka GRMZM2G397518, bHLH67}, LOC542318 (sugary 1) [NCBI Gene 542318] {aka GRMZM2G138060, isoamylase, su1, sugary1}
- **Diseases:** drought (MESH:C536747), NBS-19 (MESH:D049932)
- **Chemicals:** CO2 (MESH:D002245), humic acids (MESH:D006812), 18O (-), NaOCl (MESH:D012973), l-serine (MESH:D012694), phosphoric acid (MESH:C030242), Ti (MESH:D014025), water (MESH:D014867), apatite (MESH:D001031), glycine (MESH:D005998), NaOH (MESH:D012972), ethanol (MESH:D000431), tin (MESH:D014001), acetic acid (MESH:D019342), HCl (MESH:D006851), Oxygen (MESH:D010100), apatite carbonate (MESH:C030782), P (MESH:D010758), phosphate (MESH:D010710), carbonate (MESH:D002254), C (MESH:D002244), N (MESH:D009584)
- **Species:** Odocoileus virginianus (white-tailed deer, species) [taxon 9874], Equus caballus (domestic horse, species) [taxon 9796], Cervidae (deer, family) [taxon 9850], Oryctolagus cuniculus (domestic rabbit, species) [taxon 9986], Sylvilagus audubonii (desert cottontail, species) [taxon 30581], Antilocapra americana (pronghorn, species) [taxon 9891], Homo sapiens (human, species) [taxon 9606], Zea mays (maize, species) [taxon 4577], Zea mays subsp. mexicana (annual teosinte, subspecies) [taxon 4579], Canis lupus familiaris (dog, subspecies) [taxon 9615]

## Full text

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## Figures

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## References

140 references — full list in the complete paper: https://tomesphere.com/paper/PMC12959416/full.md

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Source: https://tomesphere.com/paper/PMC12959416