# Whole-genome sequences of the dwarf honey bee subgenus Micrapis: Apis andreniformis and Apis florea

## TL;DR

This paper provides high-quality genome sequences for two dwarf honey bee species, offering a valuable resource for future genetic studies.

## Contribution

The study presents improved and highly contiguous genome assemblies for Apis andreniformis and Apis florea using hybrid sequencing.

## Key findings

- High contig N50 values of 5.0 Mb and 4.3 Mb were achieved for A. andreniformis and A. florea, respectively.
- Genome completeness exceeded 98.5% as assessed by BUSCO scores and k-mer analyses.
- RNA sequencing enabled annotation of over 12,000 genes in each species with nearly complete proteomes.

## Abstract

The Micrapis subgenus, which includes the black dwarf honey bee (Apis andreniformis) and the red dwarf honey bee (Apis florea), remains underrepresented in genomic studies despite its ecological significance. Here, we present high-quality de novo genome assemblies for both species, generated using a hybrid sequencing approach combining Oxford Nanopore Technologies long reads with Illumina short reads. The final assemblies are highly contiguous, with contig N50 values of 5.0 Mb (A. andreniformis) and 4.3 Mb (A. florea), representing a major improvement over the previously published A. florea genome. Genome completeness assessments indicate high quality, with BUSCO scores exceeding 98.5% using the Hymenoptera database and k-mer analyses supporting base-level accuracy. Repeat annotation revealed a relatively low repetitive sequence content (∼6%), consistent with other Apis species. Using RNA sequencing data, we annotated 12,189 genes for A. andreniformis and 12,207 genes for A. florea, with ∼98% completeness in predicted proteomes. These genome assemblies provide a valuable resource for comparative and functional genomic studies, with the potential to offer new insights into the genetic basis of dwarf honey bee adaptations.