# Framework-mediated binding of foreign and self-glycans by IGHV4–34 antibodies

## TL;DR

This paper explains how IGHV4–34 antibodies bind to glycans in both cold agglutinin disease and HIV, using a unique hydrophobic patch in their framework region.

## Contribution

The study provides structural insights into how the FR1 patch of IGHV4–34 antibodies directly binds glycans, offering a new mechanistic understanding.

## Key findings

- Structures show the FR1 patch of IGHV4–34 antibodies binds high mannose glycans on HIV.
- The FR1 patch engages glycans in a non-CDR mode, suggesting a mechanism for self-antigen binding in cold agglutinin disease.
- Stereochemical similarities between HIV glycans and LacNAc support a conserved binding mechanism.

## Abstract

Cold agglutinin disease is an autoimmune condition characterised by expression of self-reactive antibodies to I/i carbohydrate blood group antigens (polymers of N-acetyllactosamine or ‘LacNAc’ units) resulting in anaemia through the agglutination and complement-mediated destruction of red blood cells. This antibody response is dominated by antibodies of the human IGHV4–34 germline, which display a unique hydrophobic patch formed by germline-encoded tryptophan and tyrosine residues at positions 7 and 25 within framework 1 (FR1). Although the requirement for conservation of this FR1 patch for binding to the I/i carbohydrate antigen has been well established, structural insights regarding the mechanistic role of the FR1 patch have remained elusive. Intriguingly, recent papers describing IGHV4–34 antibodies bound to the glycan-adorned surface of the HIV envelope glycoprotein shed light on the utility of this FR1 surface. Rather than indirectly shaping the conformation of the conventional antigen binding site as previously proposed, the structures reveal direct interfaces between the hydrophobic FR1 patch and high mannose glycans projected by HIV. Given the stereochemical similarities between these glycans and LacNAc, these structures suggest how I/i self-antigen glycans might be similarly engaged by IGHV4–34 antibodies in a framework-centric non-CDR mode and provide a rationale for the preservation of this otherwise self-reactive antibody germline.