# Thinking outside the boxes: analyzing the current landscape of popular behavioral tests for adult zebrafish

**Authors:** Allan V. Kalueff, Adam Michael Stewart, Murilo S. de Abreu, Matthew O. Parker

PMC · DOI: 10.1038/s41684-026-01694-w · Lab Animal · 2026-02-26

## TL;DR

This paper surveys how zebrafish behavior is studied and finds a need for more innovation and standardization in testing methods.

## Contribution

The study identifies overreliance on a few assays and inconsistent naming in adult zebrafish behavioral testing.

## Key findings

- Many labs rely heavily on a small number of established behavioral assays.
- Inconsistent nomenclature across labs hinders standardization.
- There is a call for innovation in adult zebrafish neurobehavioral testing.

## Abstract

Zebrafish are commonly tested in various behavioral assays. To better understand such practices, we surveyed active global zebrafish neuroscience labs, asking to rank such assays based on their perceived utility. Overreliance on few well-established assays and their inconsistent nomenclature call for further innovation and standardizing of adult zebrafish neurobehavioral testing.

## Linked entities

- **Species:** Danio rerio (taxon 7955)

## Full-text entities

- **Diseases:** aggression (MESH:D010554), sociality (OMIM:300082), hyper (MESH:D007589), brain disorders (MESH:D001927), CNS disease (MESH:D002493), anxiety (MESH:D001007), fear (MESH:C000719212)
- **Chemicals:** ZTI (-)
- **Species:** Actinopterygii (fishes, superclass) [taxon 7898], Danio rerio (leopard danio, species) [taxon 7955], Homo sapiens (human, species) [taxon 9606]

## Full text

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Source: https://tomesphere.com/paper/PMC12956589