# Integrated Microfluidic Platform for High‐Throughput Generation of Intestinal Organoids in Hydrogel Droplets

## TL;DR

A new microfluidic platform creates uniform intestinal organoids for scalable and reproducible research in drug screening and disease modeling.

## Contribution

An integrated microfluidic system for high-throughput, reproducible generation of intestinal organoids in hydrogel droplets.

## Key findings

- The platform enables uniform encapsulation of intestinal stem cells in hydrogel microparticles.
- Healthy and tumor-derived organoids were successfully cultured with high homogeneity and reproducibility.
- The system supports efficient transfer of microparticles to aqueous media for downstream applications.

## Abstract

Organoid research offers valuable insights into human biology and disease, but reproducibility and scalability remain significant challenges, particularly for epithelial organoids. Here, we present an integrated microfluidic platform that addresses these limitations by enabling high‐throughput generation of uniform hydrogel microparticles embedded with primary‐derived human adult intestinal stem cells. Our platform includes a cell distribution system for homogeneous cell encapsulation and a microfluidic oil‐removal module for efficient particle transfer to aqueous media. We demonstrate the successful culture and differentiation of both healthy‐ and tumor‐derived intestinal organoids within these microparticles, achieving high homogeneity and reproducibility. This integrated microfluidic approach holds promise for scalable and standardized organoid production, with potential applications in drug screening, disease modeling, and personalized medicine.

This integrated microfluidic platform enables high‐throughput, homogeneous encapsulation of primary human intestinal stem cells within uniform hydrogel microparticles. Featuring oil‐removal and cell‐distribution modules, the system ensures scalable production and reproducible culture of healthy and tumor‐derived organoids. This standardized approach offers a robust framework for advancing drug screening and disease modeling applications.