# Highly fluorescent method for ultra-sensitive estimation of delafloxacin in human plasma: greenness assessment

## TL;DR

A new eco-friendly method for detecting delafloxacin in human plasma with high sensitivity and precision is developed.

## Contribution

A novel spectrofluorimetric method using a micellar fluorescent complex for ultra-sensitive delafloxacin detection in plasma.

## Key findings

- The method detects delafloxacin in the range of 2.0–120.0 ng mL–1 with a detection limit of 0.6 ng mL–1.
- The method is validated for accuracy and precision in both tablets and plasma samples.
- The approach aligns with green chemistry principles due to its eco-friendly properties.

## Abstract

A simple, rapid, sensitive, and reliable spectrofluorimetric approach has been developed for the analysis of delafloxacin (DEL) in human plasma. The proposed method is based on the formation of a micellar fluorescent complex through the interaction of DEL with 2% sodium dodecyl sulfate (SDS) using a phosphate buffer (pH 4.2), producing a pronounced emission at 470 nm upon excitation at 380 nm. The experimental conditions that affected the one-pot synthesis were investigated and optimized. The calibration curve exhibited a linear relationship over a concentration range of 2.0–120.0 ng mL–1, with detection (LOD) and quantitation limits (LOQ) 0.6 and 1.9 ng mL–1, respectively. The designed approach was validated following the ICH and FDA guidelines, producing a precise analysis of DEL in its tablets and plasma samples with high recovery percentage. The results provide that the method possesses excellent eco-friendly properties, highlighting its strong compatibility with the principles of green chemistry.

The online version contains supplementary material available at 10.1186/s13065-026-01746-9.