# The complete mitochondrial genome of Dunaliella salina CS-265: insights into gene content and phylogenetic placement

**Authors:** A. K. Lisa, W. G. Reeve, D. W. Laird, A. Chopra, N. R. Moheimani

PMC · DOI: 10.1080/23802359.2026.2635789 · Mitochondrial DNA. Part B, Resources · 2026-03-02

## TL;DR

This paper presents the full mitochondrial genome of a salt-tolerant alga from Australia, revealing insights into its gene structure and evolution.

## Contribution

The study provides the first complete mitochondrial genome of Dunaliella salina CS-265 and highlights its unique gene content and fragmentation.

## Key findings

- The genome is a 30,073 bp circular DNA molecule encoding seven protein-coding genes, nine rRNAs, and three tRNAs.
- Four core genes are fragmented by multiple introns, while ATP synthase and ribosomal protein genes are absent.
- The genome reflects reductive evolution in Dunaliella mitochondria and adds to understanding of halotolerant green algae.

## Abstract

We report the complete mitochondrial genome of the halotolerant green alga Dunaliella salina CS-265, isolated from a hypersaline lake in central Australia. The genome is a circular DNA molecule of 30,073 bp, encoding seven protein-coding genes, nine rRNAs, and three tRNAs. Four core genes (cox1, cob, nad1, and nad5) are fragmented by multiple introns, whereas others remain intact. The absence of ATP synthase subunits and ribosomal protein genes reflects ongoing reductive evolution in Dunaliella mitochondria. This genome adds a new organellar resource from an Australian isolate, complementing previous studies and providing further insight into mitochondrial genome dynamics in halotolerant green algae.

## Linked entities

- **Genes:** COX1 (cytochrome c oxidase subunit I) [NCBI Gene 4512], MMAB (metabolism of cobalamin associated B) [NCBI Gene 326625], nad1 (NADH dehydrogenase subunit 1) [NCBI Gene 800323], nad5 (NADH dehydrogenase subunit 5) [NCBI Gene 800311]
- **Species:** Dunaliella salina (taxon 3046)

## Full-text entities

- **Genes:** trnM [NCBI Gene 11541819], cox1 [NCBI Gene 8139880], nad4 [NCBI Gene 8139881], trnQ [NCBI Gene 11541784], nad6 [NCBI Gene 8139876], trnW [NCBI Gene 11541758], nad5 [NCBI Gene 8139859], rrnS [NCBI Gene 11541775], cob [NCBI Gene 8139873], nad1 [NCBI Gene 8139877], rrnL [NCBI Gene 11541777], nad2 [NCBI Gene 8139866]
- **Chemicals:** salt (MESH:D012492), carotenoid (MESH:D002338), CTAB (MESH:D000077286), ATP synthase subunits (-), beta-carotene (MESH:D019207)
- **Species:** Pleodorina starrii (species) [taxon 330485], Chlamydomonas moewusii (species) [taxon 3054], Dunaliella (genus) [taxon 3044], Chlamydomonas reinhardtii (species) [taxon 3055], Chlorosarcinopsis eremi (species) [taxon 332213], Eudorina elegans (species) [taxon 47282], Dunaliella salina (species) [taxon 3046], Chlorophyta (green algae, phylum) [taxon 3041], Chlamydomonas sp. (species) [taxon 2812568]
- **Cell lines:** NC_026573.1 — Homo sapiens (Human), Transformed cell line (CVCL_1874), MKA.28 — Oryctolagus cuniculus (Rabbit), Transformed cell line (CVCL_6E94), CS-265 — Mus musculus (Mouse), Hybridoma (CVCL_J809)

## Full text

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## Figures

3 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12954796/full.md

## References

31 references — full list in the complete paper: https://tomesphere.com/paper/PMC12954796/full.md

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Source: https://tomesphere.com/paper/PMC12954796