# A trio-binned, haplotype-resolved genome sequence of the zebrafish, Danio rerio Hamilton 1822, SAT strain

**Authors:** Kerstin Howe, Arang Rhie, Sergey Koren, Elisabeth Busch-Nentwich, Shane A. McCarthy, Jonathan M. D. Wood, Michelle Smith, Gene Myers, Karen Oliver, Annabel Whibley, Tim Martin Knutsen, Jayan Duminda M Senevirathna

PMC · DOI: 10.12688/wellcomeopenres.25185.1 · Wellcome Open Research · 2025-12-22

## TL;DR

This paper provides a detailed, haplotype-resolved genome sequence of a zebrafish SAT strain individual.

## Contribution

The study presents the first trio-binned, haplotype-resolved genome assemblies for the zebrafish SAT strain.

## Key findings

- The paternal haplotype genome is 1,354.1 megabases long.
- The maternal haplotype genome is 1,360.5 megabases long.
- The mitochondrial genome is 16.6 kilobases in length.

## Abstract

We present the trio-binned, haplotype-resolved genome assemblies (released in 2020) of both haplotypes of an individual of the
Danio rerio SAT strain, a cross between Tuebingen (maternal) and AB (paternal) strains (zebrafish; Chordata; Actinopteri; Cypriniformes; Cyprinidae). The genome sequence of the paternal haplotype (fDreABH1) is 1,354.1 megabases long, while the genome sequence of the maternal haplotype (fDreTuH) is 1,360.5 megabases long. Most of the assembly is scaffolded into 25 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.6 kilobases in length.

## Linked entities

- **Species:** Danio rerio (taxon 7955), Mus musculus (taxon 10090)

## Full-text entities

- **Chemicals:** DHAB (-), agarose (MESH:D012685), Nucleotide (MESH:D009711)
- **Species:** Homo sapiens (human, species) [taxon 9606], Danio rerio (leopard danio, species) [taxon 7955], Actinopterygii (fishes, superclass) [taxon 7898]

## Full text

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## Figures

4 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12954400/full.md

## References

17 references — full list in the complete paper: https://tomesphere.com/paper/PMC12954400/full.md

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Source: https://tomesphere.com/paper/PMC12954400