# Aspergillus conicus Endophyte Improves the Development of Eucalyptus camaldulensis Seedlings In Vitro

## TL;DR

A fungus called Aspergillus conicus helps Eucalyptus camaldulensis seedlings grow faster and better in lab conditions.

## Contribution

This study identifies A. conicus as a beneficial endophyte for E. camaldulensis seedling development.

## Key findings

- Inoculated seedlings showed faster development, more leaves, and greater biomass.
- Seeds treated with A. conicus filtrate had higher germination rates and less fungal contamination.
- The fungus colonized roots, stems, and leaves, indicating a strong endophytic relationship.

## Abstract

Fungi of the genus Aspergillus promote plant growth and resistance, enhance nutrient uptake, protect plants against pathogens, and increase tolerance to environmental stress. We examined the symbiosis between Aspergillus conicus and seedlings of Eucalyptus camaldulensis, a forest species widely grown in Brazil for its valuable wood and resilience. The fungus was identified as an endophyte of E. camaldulensis seeds grown in Murashige and Skoog basal medium. We observed that inoculated seedlings developed faster than those without the fungus. In xerophilic medium, A. conicus produced abundant spores. Analysis of the internal transcribed spacer region grouped the isolate with other A. conicus species. Seedlings grown on Murashige and Skoog medium with fungal fragments showed significant shoot growth, more leaves, and greater biomass than uninoculated seedlings. Seeds immersed in A. conicus filtrate for 24 h showed less contamination by other fungi and a higher germination rate than those in the control group. Indole acetic acid production was below the detection limit. The fungus was endophytic, extensively colonizing the roots and present in the stems and leaves of inoculated plants. We investigated the implications of this fungal association with E. camaldulensis seedlings and highlighted its potential benefits for plant growth and development.