# Comparative Analysis of Akerma and Bouchouk Guergour Olive Oils Varieties: Physicochemical Analysis, Quality, and Bioactivity Evaluation

**Authors:** Samir Sahli, Nabila Souilah, Hamdi Bendif, Amar Benmahammed, Saliha Dahamna, Ramazan Erenler, Walid Elfalleh, İbrahim Demirtaş, Fehmi Boufahja, Stefania Garzoli

PMC · DOI: 10.1002/cbdv.202502264 · Chemistry & Biodiversity · 2026-03-02

## TL;DR

This study compares two olive varieties, Akerma and Bouchouk Guergour, analyzing their ripening, oil yield, quality, and wound healing properties.

## Contribution

The study introduces new insights into the ripening dynamics and bioactivity of two specific olive varieties.

## Key findings

- Akerma olive oil has lower acidity and slower ripening compared to Bouchouk Guergour.
- Both oils showed significant wound healing effects, especially in their black stages.
- Oil yield peaks at different ripening stages for each variety, affecting harvest timing.

## Abstract

The current study investigated the dynamic of the ripening process, oil yield, physicochemical properties, and wound healing activity of two olive varieties, namely Akerma and Bouchouk Guergour. The ripening index revealed that Akerma matured at a slower rate compared to the Bouchouk Guergour variety. The oil yield peaked at 12.71% for the Akerma variety at the spotted stage and 19.56% for Bouchouk Guergour at the black stage, suggesting earlier harvesting time in short‐growing seasons for the former oil tree. Physicochemical analyses showed compliance with International Olive Council standards, with Akerma exhibiting lower acidity compared to Bouchouk Guergour variety. The peroxide value increased with ripening, indicating higher oxidative susceptibility in mature oils. Gas chromatography‐mass spectrometry (GC‐MS) analysis revealed a high content of monounsaturated fatty acids (MUFAs), particularly oleic acid. Notably, in vivo, evaluations demonstrated significant wound healing effects, with both oils achieving complete recovery by Day 28, particularly in their black stages, and showcasing anti‐inflammatory properties. These findings underscore the importance of varietal selection and optimal harvest timing to enhance oil quality, yield, and therapeutic potential. This research provides valuable insights for olive growers, facilitating the maximization of production and promoting health benefits associated with high‐quality olive oils.

## Linked entities

- **Chemicals:** oleic acid (PubChem CID 445639)

## Full-text entities

- **Diseases:** Mortality (MESH:D003643), inflammatory drug (MESH:D000081015), edema (MESH:D004487), Ear edema (MESH:D004427), pain (MESH:D010146), Inflammatory (MESH:D007249), Burns (MESH:D002056)
- **Chemicals:** Na2S2O3 (MESH:C017717), Oil (MESH:D009821), Fatty Acid (MESH:D005227), hydrocarbons (MESH:D006838), MUFA (MESH:D005229), tocopherols (MESH:D024505), PGE1 (MESH:D000527), phenols (MESH:D010636), TFA (MESH:D014269), starch (MESH:D013213), Linoleic acid methyl ester (MESH:C005575), Peroxide (MESH:D010545), potassium iodide (MESH:D011193), PUFA (MESH:D005231), hexane (MESH:D006586), Palmitoleic acid (MESH:C008757), Olive Oil (MESH:D000069463), eicosanoids (MESH:D015777), Bouchouk Guergour variety oil (-), DHA (MESH:C027493), Palmitic acid methyl ester (MESH:C019012), diethyl ether (MESH:D004986), hydrogen (MESH:D006859), oleuropein (MESH:C002769), alcohol (MESH:D000438), Oleic acid (MESH:D019301), Iodine (MESH:D007455), KOH (MESH:C029943), lipid (MESH:D008055), KI (MESH:C066186), Docosahexaenoic acid (MESH:D004281), Palmitic acid (MESH:D019308), chloroform (MESH:D002725), phenolphthalein (MESH:D020113), Vaseline (MESH:D010577), polyphenols (MESH:D059808), divinylbenzene (MESH:C004985), carbon (MESH:D002244), triglycerides (MESH:D014280), omega-3 fatty acid (MESH:D015525), sodium benzoate (MESH:D020160), Biafine (MESH:C423724), xylene (MESH:D014992), stainless-steel (MESH:D013193), O2 (MESH:D010100), T (MESH:D014316), methanol (MESH:D000432), C18:2 n-6 (MESH:D019787), palmitoleic acid methyl ester (MESH:C068012), fat (MESH:D005223), hydroxytyrosol (MESH:C005975), iodine monochloride (MESH:C009813), FAME (MESH:C508762), cholesterol (MESH:D002784), Trans fatty acid (MESH:D044242), ethanol (MESH:D000431), Trolamine (MESH:C009546), oleic acid methyl ester (MESH:C005576), acetic acid (MESH:D019342), silicone (MESH:D012828)
- **Species:** Homo sapiens (human, species) [taxon 9606], Rattus norvegicus (brown rat, species) [taxon 10116], Mus musculus (house mouse, species) [taxon 10090], Olea (olives, genus) [taxon 4145], Olea europaea (common olive, species) [taxon 4146]

## Full text

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## Figures

15 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12953217/full.md

## References

51 references — full list in the complete paper: https://tomesphere.com/paper/PMC12953217/full.md

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Source: https://tomesphere.com/paper/PMC12953217