# Genomic diversity and host-specificity in Corynebacterium pseudotuberculosis using comparative population genomics

## TL;DR

This study explores the genomic diversity of Corynebacterium pseudotuberculosis and finds that host adaptation is driven by small genetic changes in core genes, not by acquiring new virulence genes.

## Contribution

The study reveals that host specificity in C. pseudotuberculosis is due to SNP variations in core metabolic genes, not accessory genes.

## Key findings

- Genome architecture is highly conserved with a closed pangenome and minimal accessory gene variation.
- Host adaptation is linked to allelic variants in core genes related to amino-acid biosynthesis and peptide transport.
- Virulence and AMR genes are largely conserved, with only a few exceptions in specific isolates.

## Abstract

Corynebacterium pseudotuberculosis is a facultative intracellular pathogen responsible for chronic infections in livestock, primarily small ruminants and horses, with occasional zoonotic transmission. To investigate the genomic diversity, evolutionary stability, and host adaptation of this species, we analyzed 788 high-quality genomes representing isolates from diverse hosts, geographic regions, and time periods. Comparative population genomics revealed remarkably conserved genome architecture, supporting a closed pangenome with minimal accessory gene variation. Virulence and antimicrobial resistance (AMR) screening across multiple databases confirmed the universal presence of phospholipase D (pld) and the absence of major horizontally acquired AMR determinants, except for APH(3’)-IIa, TEM-116, and APH(3’)-IIIa in a few goat isolates from Brazil. Distinct metabolic features between biovars were conserved, notably nitrate reduction and molybdenum cofactor biosynthesis in biovar equi. However, gene presence/absence alone did not explain host specificity. Instead, machine learning applied to 8,028 core-genome SNPs identified allelic variants associated with host origin, particularly in genes linked to amino-acid biosynthesis and peptide transport (Opp system). These findings demonstrate that host adaptation in this species is driven by fine-scale SNP variation within core metabolic pathways, rather than acquisition of classical virulence or resistance genes, highlighting the species’ exceptional genomic stability and narrow evolutionary flexibility.