# Enhancing soybean (Glycine max L.) yield and quality through optimized weed-free periods and sowing techniques

**Authors:** Muhammad Awais Arshad, Rana Nadeem Abbas, Rania Baloch, Ali Ahmad, Usman Zulfiqar, Fasih Ullah Haider, Hossam S. El-Beltagi, Mashael Daghash Alqahtani, P. V. Vara Prasad

PMC · DOI: 10.3389/fpls.2026.1700878 · Frontiers in Plant Science · 2026-02-16

## TL;DR

This study finds that keeping soybean fields weed-free for 25-40 days after planting improves yield and quality, especially when using flat sowing.

## Contribution

The study identifies the optimal weed-free period and sowing method for maximizing soybean yield and quality under non-GMO conditions.

## Key findings

- Maintaining a weed-free period of 25–40 days after sowing maximized soybean grain yield and improved seed protein and oil content.
- Flat sowing outperformed bed sowing due to better canopy closure and reduced weed pressure during the critical weed-free period.
- Weed competition throughout the season caused 57–62% yield loss in flat sowing and 58–60% in bed sowing.

## Abstract

Soybean (Glycine max L.) yield is sensitive to early weed competition, yet optimal weed control timing and sowing methods remain unclear. Although early-season weed interference is widely recognized as detrimental, critical knowledge gaps persist regarding the precise weed-free period required and how sowing configuration affects weed dynamics and crop performance. Hence, this study aimed to clarify the effects of different weed-free durations and sowing methods (flat vs. bed) on soybean growth, yield, and seed quality. Over two years, a split-plot randomized block design with three replications was used to examine 16 treatments, including two sowing methods (bed sowing and flat sowing) with weed competition and weed-free periods of up to 25, 40, and 55 days after sowing (DAS), along with full-season competition and a weed-free period. Data were recorded to evaluate weed density, growth, yield, and quality parameters, including protein and oil content. Results showed that maintaining a weed-free window between 25–40 DAS was critical to minimizing yield losses. Weed competition throughout the season caused 57–62% yield loss in flat sowing and 58–60% in bed sowing, while weed-free management during 25–40 DAS maximized grain yield and improved seed protein and oil content. Flat sowing consistently outperformed bed sowing due to better canopy closure, reduced weed pressure, and improved resource capture during the critical weed-free period. In conclusion, maintaining a weed-free environment during the 25–40 DAS window, particularly under flat sowing, optimizes soybean growth, yield, and quality under non-GMO conditions. Future research should prioritize integrated, site-specific weed management strategies and assess their long-term impacts on economic returns, soil health, and environmental sustainability.

## Full-text entities

- **Genes:** FT2A (protein FLOWERING LOCUS T) [NCBI Gene 100814951] {aka E9, FT, FT3, FTL3, GmFT2a}, WRKY [NCBI Gene 100127393], MYB [NCBI Gene 547568]
- **Chemicals:** carbon (MESH:D002244), nitrogen (MESH:D009584), fat (MESH:D005223), phenolic acids (MESH:C017616), terpenoids (MESH:D013729), gibberellin (MESH:D005875), carbohydrates (MESH:D002241), oil (MESH:D009821), Seed oil (-), jasmonic acid (MESH:C011006), caffeic acid (MESH:C040048)
- **Species:** Trianthema portulacastrum (species) [taxon 3548], Glycine max (soybean, species) [taxon 3847], Tribulus terrestris (species) [taxon 210369], Parthenium hysterophorus (species) [taxon 183063], Convolvulus arvensis (species) [taxon 4123], Cyperus rotundus (species) [taxon 512623]

## Full text

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## Figures

7 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12951636/full.md

## References

84 references — full list in the complete paper: https://tomesphere.com/paper/PMC12951636/full.md

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Source: https://tomesphere.com/paper/PMC12951636