# Multi‐Solvent Suppression Ultrafast 2D COSY for High‐Throughput Wine Screening

## TL;DR

A new NMR method called iuf-COSY was developed to quickly analyze wine metabolites by suppressing water and ethanol signals, improving identification and quality control.

## Contribution

An ultrafast 2D COSY method with multi-solvent suppression for high-throughput wine metabolite analysis in ~20 minutes.

## Key findings

- The method suppresses water and ethanol signals, revealing hidden metabolite peaks in wine.
- It produces 2D COSY spectra in ~20 minutes, suitable for high-throughput analysis.
- The approach aids in metabolite identification and discrimination for wine studies.

## Abstract

Nuclear magnetic resonance (NMR) is a powerful analytical tool for wine analysis to identify and quantify a metabolite composition. However, a limiting factor of 1D 1H NMR spectroscopy is the overlap of signals in complex mixtures. While conventional 2D NMR methods disperse the signals over two dimensions, they are associated with long experiment times. In the case of wine, interesting metabolites are also often masked by the large water and ethanol peaks. To improve wine analysis by NMR, a method that uses the advantages of 2D NMR while suppressing solvent signals and being within the timeframe of 1D NMR is highly desirable. Interleaved ultrafast COSY (iuf‐COSY) offers a possibility for fast acquisition of a 2D spectrum and has been demonstrated as a powerful tool in metabolomics studies, as a complement to 1D NMR methods. Here, the iuf‐COSY experiment has been adapted to suppress water and ethanol signals by using a shaped pulse and a NOESY block. This approach efficiently suppresses solvent signals and gives a 2D COSY spectrum of wine in approximately 20 min. Important metabolites that originally were covered by solvent signals could be annotated, while minimal interleaving artefacts were observed. This is an efficient method to acquire a COSY spectrum of a wine sample, which can aid with the identification and discrimination of metabolites in future wine studies through additional cross peaks, while working within a high‐throughput time scale. This might be particularly interesting in the field of wine metabolomics, quality control, authenticity and fraud.

An interleaved ultrafast COSY with multi‐solvent suppression was developed for wine analysis to overcome limitations often associated with 1D 1H NMR (signal overlap) and conventional 2D NMR (long acquisition times) methods. Within an acquisition time of ~20 min, it revealed hidden metabolite peaks and cross peaks for improved annotation and quantification. This method offers new possibilities for wine metabolomic studies, wine authenticity, quality control and fraud detection.