# 38‐Marker Full‐Spectrum Flow Cytometry Panel for the Comprehensive Profiling of γδ T Cells in Human Blood and Lymphoid Tissues

## TL;DR

This paper introduces a 38-marker flow cytometry panel for detailed analysis of human γδ T cells in blood and lymphoid tissues.

## Contribution

The novel contribution is a comprehensive 38-marker panel for high-resolution profiling of γδ T cell subsets.

## Key findings

- The panel enables robust identification of rare, tissue-adapted γδ T cell subsets.
- It provides high-resolution profiling across blood and lymphoid tissues.

## Abstract

This is an update to the Guidelines for the Use of Flow Cytometry and Cell Sorting in Immunological Studies (Third Edition), Chapter 12C, by Cossarizza et al. A 38‐marker full‐spectrum flow cytometry panel enabling high‐resolution profiling of human γδ T cells across blood and lymphoid tissues, including robust identification of rare, tissue‐adapted subsets.