# DNA Vaccination Against Macrophage Migration Inhibitory Factor Protecting Mice From Experimental Pancreatitis

## TL;DR

A DNA vaccine targeting MIF reduces inflammation and protects mice from experimental pancreatitis.

## Contribution

A novel DNA vaccine strategy using auto-MIF antibodies to treat pancreatitis is demonstrated in mice.

## Key findings

- MIF-DNA vaccination increased MIF antibody titers and reduced MIF levels in mice with pancreatitis.
- The vaccine suppressed inflammatory markers like IL-1β and MCP-1 in the pancreas and serum.
- Pancreatic HSP70 levels were upregulated in vaccinated mice, indicating cytoprotective effects.

## Abstract

Objective

Macrophage migration inhibitory factor (MIF) has a pivotal role in the development of gastroenterological diseases, including pancreatitis. In this study, we aimed to explore the effect of deoxyribonucleic acid (DNA) vaccination producing an auto-MIF antibody on experimental pancreatitis and to provide additional evidence that MIF affects the development of pancreatitis.

Methods

Mice were treated with an MIF-DNA vaccine by introducing oligonucleotides encoding a helper T epitope into the cDNA sequence of murine MIF by in vivo electroporation. Thereafter, experimental pancreatitis was induced by seven repeated intraperitoneal injections of cerulein (50 μg/kg). Histological findings were evaluated in the pancreas. The levels of MIF, monocyte chemoattractant protein-1 (MCP-1), IL-1β, and heat shock protein 70 (HSP70) were analyzed with enzyme-linked immunosorbent assay (ELISA).

Results

The titer of MIF antibody was increased in the serum of mice 8 weeks after the treatment with MIF-DNA vaccine. In cerulein-induced pancreatitis, the histological findings in the pancreas were ameliorated in MIF-DNA vaccinated mice. The serum levels of MIF were lower in MIF-DNA vaccinated mice than those in mock-treated mice with pancreatitis. The increases in the serum and pancreatic levels of IL-1β and the serum level of MCP-1 were suppressed in MIF-DNA-vaccinated mice given cerulein. Furthermore, the pancreatic HSP70 level was upregulated in MIF-DNA-vaccinated mice given cerulein.

Conclusion

MIF-DNA vaccination protected mice from cerulein-induced pancreatitis via anti-inflammatory and cytoprotective effects. MIF-DNA vaccination may be an additional option for the treatment of pancreatitis.