# Safety and efficacy of a feed additive consisting of l‐isoleucine produced with Corynebacterium glutamicum CCTCC M 2022764 for all animal species (Innobio Europe B.V.)

## TL;DR

The paper evaluates the safety and effectiveness of l-isoleucine produced by a specific bacteria for use in animal feed.

## Contribution

It confirms the safety of l-isoleucine for non-ruminant animals and highlights concerns about its use in drinking water.

## Key findings

- The additive is safe for production and target species when used appropriately.
- Concerns exist regarding its use in drinking water.
- It is effective for non-ruminants but needs protection for ruminants.

## Abstract

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of l‐isoleucine produced by fermentation with a non‐genetically modified strain of Corynebacterium glutamicum (CCTCC M 2022764) as a nutritional feed additive for use in feed and water for drinking for all animal species. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the additive does not give rise to any safety concern regarding the production strain. The use of l‐isoleucine produced with C. glutamicum CCTCC M 2022764 is considered safe for the target species when supplemented in appropriate amounts to the diet according to the nutritional needs of the target species. The FEEDAP Panel has concerns on the use of l‐isoleucine in water for drinking. The use of l‐isoleucine produced with C. glutamicum CCTCC M 2022764 as a feed additive is considered safe for the consumer and the environment. The FEEDAP Panel cannot conclude on the potential of the additive to be an irritant to skin and/or eyes and to be a potential skin sensitiser. The feed additive consisting of l‐isoleucine produced by fermentation with C. glutamicum CCTCC M 2022764 is regarded as an effective source of the amino acid l‐isoleucine for all non‐ruminant species. For the supplemental l‐isoleucine to be as efficacious in ruminants as in non‐ruminant species, it should be protected from ruminal degradation.