# Microstructural and Physical Properties of High‐Protein, High‐Overrun Frozen Desserts

**Authors:** Samantha R. VanWees, Scott A. Rankin, Richard W. Hartel

PMC · DOI: 10.1111/1750-3841.70944 · Journal of Food Science · 2026-02-26

## TL;DR

This paper explores how different proteins affect the texture and structure of high-protein frozen desserts like ice cream.

## Contribution

The study identifies how specific proteins and additives influence the microstructure and physical properties of high-protein frozen desserts.

## Key findings

- Adding 0.15% mono- and diglycerides reduced ice crystal and air cell size in frozen desserts.
- Fat destabilization increased resistance to collapse during melting in desserts with milk protein concentrate and whey protein isolate.
- The interfacial activity of proteins and additives influenced the formation and stabilization of the frozen dessert structure.

## Abstract

Ice cream and frozen desserts fortified with protein often have undesirable physical and textural properties despite their increased nutritional value, and are susceptible to shrinkage during storage. The effects of dairy protein structure on structural and physical properties of the mix and frozen product were identified by studying frozen dessert mixes formulated to contain 6% milk protein concentrate, sodium caseinate, or whey protein isolate. The addition of 0.15% mono‐ and diglycerides decreased the mean ice crystal and air cell size in all frozen desserts and increased the degree of fat destabilization in frozen desserts made with milk protein concentrate and whey protein isolate, providing resistance to collapse during melting. The interfacial activity of serum proteins, casein micelles, nonmicellar casein, and mono‐ and diglycerides was essential to sequential formation and stabilization of structure, especially with regard to the emulsified fat globule membrane composition and formation of destabilized fat network during freezing. The correlation between rate of drip‐through during melting and degree of fat destabilization was independent of protein source, demonstrating how mix ingredient functionality influenced the co‐development of ice, air, fat, and serum phase structures in the frozen dessert, which ultimately governed the physical properties of the frozen dessert.

With the continued interest in high‐protein products, this work demonstrates the potential for selectively choosing type of protein to enhance physicochemical and microstructural properties of ice cream and frozen dairy desserts.

## Linked entities

- **Proteins:** LOC105090951 (alpha-S2-casein)
- **Chemicals:** mono- and diglycerides (PubChem CID 3086206)

## Full-text entities

- **Diseases:** MPC (MESH:D016269), Interfacial Tension (MESH:D018781)
- **Chemicals:** NaCN (MESH:D012966), pentanol (MESH:D000439), water (MESH:D014867), polysaccharides (MESH:D011134), lactose (MESH:D007785), carrageenan (MESH:D002351), sugars (MESH:D000073893), proline (MESH:D011392), fat (MESH:D005223), Ice (MESH:D007053), locust bean gum (MESH:C017471), sucrose (MESH:D013395), guar gum (MESH:C007894), oil (MESH:D009821), amino acids (MESH:D000596), sulfur (MESH:D013455), MPC (-)
- **Mutations:** C) for 6, C -2 C

## Full text

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## Figures

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## References

68 references — full list in the complete paper: https://tomesphere.com/paper/PMC12946691/full.md

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Source: https://tomesphere.com/paper/PMC12946691