# Precision RNAi in Tomato Using Synthetic Trans‐Acting Small Interfering RNAs Derived From Minimal Precursors

## TL;DR

Researchers developed a new RNAi method in tomatoes using minimal precursors, enabling efficient gene silencing and virus resistance without transgenes.

## Contribution

A novel syn-tasiRNA platform using minimal precursors for transgene-free, efficient gene silencing in tomato.

## Key findings

- Minimal precursors effectively produce functional syn-tasiRNAs in transgenic and transient systems.
- The method induces robust gene silencing and provides resistance to tomato spotted wilt virus.
- Syn-tasiR-VIGS can be applied transgene-free via crude extract delivery for gene silencing.

## Abstract

RNA interference (RNAi) is a highly conserved gene silencing mechanism regulating gene expression at transcriptional and post‐transcriptional levels in plants. Synthetic trans‐acting small interfering RNAs (syn‐tasiRNAs) have emerged as powerful tools for highly specific and efficient gene silencing. However, their application in crops has been constrained by the need for transgene integration and the relatively long length of TAS‐derived precursors. Here, we developed a novel syn‐tasiRNA platform for
Solanum lycopersicum
(tomato) based on minimal precursors targeted by endogenous SlmiR482b microRNA. These minimal precursors, comprising only a 22‐nt miRNA target site, an 11‐nt spacer, and the syn‐tasiRNA sequence(s), effectively produced functional syn‐tasiRNAs in both transgenic and transient virus‐induced gene silencing (syn‐tasiR‐VIGS) systems. To facilitate their broader application, we engineered a series of vectors for high‐throughput cloning and efficient syn‐tasiRNA expression from SlmiR482b‐based minimal precursors in tomato. Our results show that minimal precursors induce robust gene silencing of endogenous tomato genes and confer antiviral resistance to the economically important tomato spotted wilt virus. Furthermore, we show that syn‐tasiR‐VIGS can be applied in a transgene‐free manner through crude extract delivery, leading to efficient silencing of endogenous genes. This study establishes minimal syn‐tasiRNA precursors as a versatile and efficient tool for precision RNAi in tomato, with applications in functional genomics and crop improvement.