# Comparative Evaluation of AmnioGuard and Advanced Platelet-Rich Fibrin When Combined With NovaBone Putty in the Regeneration of Human Periodontal Infrabony Defects: Protocol for a Randomized Controlled Clinical Trial

## TL;DR

This study compares two treatments for periodontal tissue regeneration in chronic periodontitis patients.

## Contribution

The study introduces a novel comparison of AmnioGuard and A-PRF with NovaBone Putty in periodontal regeneration.

## Key findings

- AmnioGuard with NovaBone Putty may improve clinical outcomes in periodontal regeneration.
- The study will evaluate clinical and radiographic parameters over six months.
- Results are expected to show better healing with AmnioGuard and NovaBone Putty.

## Abstract

Periodontitis causes progressive attachment loss, which in turn causes tooth mobility and, ultimately, tooth loss. Regenerating and rebuilding the lost periodontal tissue is the goal of periodontal regenerative surgery. Regeneration using AmnioGuard, advanced platelet-rich fibrin (A-PRF), and NovaBone Putty has been demonstrated to be successful in increasing clinical attachment level and decreasing probing pocket depth.

This study aims to compare and evaluate the effectiveness of AmnioGuard and A-PRF membrane in combination with NovaBone Putty in the regeneration of human periodontal infrabony defects.

A total of 24 patients will be selected and divided into 2 groups: group A (n=12, 50%) and group B (n=12, 50%). Group A will receive treatment with NovaBone Putty and AmnioGuard, whereas group B will receive treatment with autologous A-PRF and NovaBone Putty. Plaque index, clinical attachment level, probing pocket depth, complete mouth bleeding score, and intraoral periapical radiographs will be statistically analyzed. Clinical and radiographic features will be evaluated before surgery and 6 months following the procedure.

Recruitment began on September 16, 2025, and is ongoing. As of manuscript submission, 4 participants have been enrolled. Results are expected to be published in 2027. All the clinical measurements will be recorded after 6 months. It can be anticipated that AmnioGuard membrane with NovaBone Putty will provide additional benefits in the management of chronic periodontitis by promoting better healing and clinical outcomes.

Within the constraints of this study, the AmnioGuard membrane combined with NovaBone Putty may be more effective in the treatment of chronic periodontitis.