# Molecular Identification and Drug Sensitivity Test of Acinetobacter lwoffii from Cynomolgus Monkey with Peritonitis

## TL;DR

Scientists identified Acinetobacter lwoffii from a sick monkey's peritoneal tissue and found it is sensitive to many antibiotics.

## Contribution

This is the first report of Acinetobacter lwoffii causing peritonitis in a cynomolgus monkey.

## Key findings

- The isolated strain MF080196 showed 99.8% similarity to known Acinetobacter lwoffii sequences.
- The strain was highly sensitive to 23 antibiotics, including ceftriaxone and gentamicin.
- The bacteria caused mortality in mice, confirming its pathogenicity.

## Abstract

A bacterial strain was isolated from the diseased peritoneal tissue of a dead cynomolgus monkey. It was identified as Acinetobacter lwoffii (A. lwoffii) by bacterial isolation and culture, morphological observation, physiological and biochemical examination and molecular biological methods. To our knowledge, this is the first report of the isolation of A. lwoffii from a peritonitis cynomolgus monkey. The results provide a reference for the prevention and treatment of bacterial peritonitis in experimental monkeys.

A case of peritonitis in a female cynomolgus monkey (Macaca fascicularis) caused by suspected bacterial infection was found. In order to identify the pathogenic bacteria, pathological tissues from the peritoneum of the animal were collected, and a bacterial strain designated MF080196 was isolated. Morphological observations were conducted, followed by biochemical testing and sequencing of its 16S rRNA gene. Additionally, pathogenicity test and drug sensitivity analyses were performed. The findings indicated that colonies grown on blood agar medium appeared white, circular, and raised; the bacteria were identified as Gram-negative; biochemical tests aligned with the characteristics of Acinetobacter lwoffii (A. lwoffii). PCR amplification was performed using a 16S rRNA sequence measuring 1443 bp; there was a remarkable similarity of 99.8% with previously reported sequences of A. lwoffii from various sources. The isolated strain MF080196 is capable of mortality in mice; furthermore, drug susceptibility testing revealed that the isolated strain exhibited high sensitivity to 23 antibiotics, including ceftriaxone, gentamicin, and levofloxacin. The pathogen isolated from the peritoneal tissue of the dead cynomolgus monkey was identified as A. lwoffii, which demonstrated sensitivity to most antibiotics tested in this study. This research provides significant insights for preventing and treating bacterial diseases in non-human primates.