# Aflatoxins and Human Health: Global Exposure, Disease Burden, and One Health Strategies

## TL;DR

Aflatoxins, especially aflatoxin B1, pose serious health risks globally, with exposure levels varying widely and new technologies being explored to reduce their impact.

## Contribution

This review highlights global aflatoxin exposure patterns and introduces new technologies for detection and mitigation within a One Health framework.

## Key findings

- Aflatoxin–albumin/lysine adducts are detected in up to 100% of populations in parts of Africa and Asia.
- Aflatoxins contribute to cancer through DNA adducts, oxidative stress, and immune effects.
- Exposure remains high in many regions despite regulatory standards, and may be increasing in low-exposure countries.

## Abstract

Mycotoxin contamination represents a major public health and economic burden worldwide. Aflatoxins, particularly aflatoxin B1, are the most detrimental for human health. In this review, we discuss the sources of exposure and geographic distribution. The prevalence of aflatoxin–albumin/lysine adduct detection in humans varies dramatically across the world, from 0% reported in two European studies to up to 100% reported in studies from parts of Africa and Asia. We also summarize the disease outcomes that aflatoxins are associated with in humans. We focus particularly on cancer outcomes, which aflatoxins can cause through mutagenic DNA adducts, oxidative stress, mitochondrial dysfunction, immune effects, and epigenetic changes. Synergy with hepatitis B virus and potentially with other mycotoxins can also increase risk. Minimization of aflatoxin exposure requires an integrative approach, beginning at the farm level and continuing through pre-harvest, post-harvest, storage, and the consumer level. New developments in technology, such as electrochemical biosensors and artificial intelligence algorithms, are being piloted and could help improve detection and decontamination efforts. Further, new tests for aflatoxin exposure in humans (e.g., blood spot assays) could assist biomonitoring efforts. Despite regulatory standards in most countries for the maximum allowable level of aflatoxins in food products and animal feed, exposure remains high in many parts of the world and might be increasing even in countries with historically low exposure. Integration of these tools in a One Health framework is essential to reduce the current and future burden of aflatoxin-related disease.