Comprehensive Genome-Wide Analysis of the AP2/ERF Transcription Factor Family and Examination of Potential Regulators CpERF140 and CpERF138 in the Biosynthesis of Lobetyolin in Codonopsis pilosula
Xiaobi Zhang, Jiaxin Zhang, Zhengqin Pan, Ping Wang, Linqiang Zhang, Jiayi Xu, Junmei Lian, Yihan Wang, Xinyue Zhang, Yan Sui, Yan Yan, Jian Zhang, Peng Di
TL;DR
This study explores how specific transcription factors regulate the production of a key compound in Codonopsis pilosula, using genome-wide analysis and hairy root cultures.
Contribution
The first genome-wide characterization of AP2/ERF transcription factors in Codonopsis pilosula and identification of CpERF140 and CpERF138 as regulators of lobetyolin biosynthesis.
Findings
175 AP2/ERF transcription factors were identified and categorized into subfamilies in Codonopsis pilosula.
MeJA treatment increased lobetyolin accumulation and activated jasmonate signaling and secondary metabolism pathways.
CpERF140 and CpERF138 were confirmed as nuclear-localized TFs with GCC-box binding affinity.
Abstract
Lobetyolin is a key bioactive compound in Codonopsis pilosula, but the existing literature has not well explored the regulatory mechanisms governing its biosynthesis. This study performed a genome-wide identification of 175 AP2/ERF transcription factors (TFs), categorizing them into the AP2, ERF, and RAV subfamilies. Promoter analysis revealed numerous hormone- and stress-responsive cis-elements, with a notable prevalence of methyl jasmonate (MeJA)-related motifs. In MeJA-treated hairy root cultures, we observed a significant increase in lobetyolin accumulation. Integrated transcriptomic and metabolomic analyses demonstrated the activation of jasmonate signaling and secondary metabolism pathways. Two MeJA-induced TFs, CpERF140 and CpERF138, were identified as key regulatory candidates. Subsequent experiments confirmed their nuclear localization and specific binding affinity for GCC-box…
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Taxonomy
TopicsPlant Gene Expression Analysis · Insect-Plant Interactions and Control · Plant biochemistry and biosynthesis