# Effects of Nitrogen Application Rates and Nitrogen Topdressing at Different Leaf Growth Stages on the Yield, Nitrogen Absorption, and Utilization of Nanjing 9108

**Authors:** Zheshu Xu, Tao Li, Jingjing Cui, Jianghui Yu, Guangyan Li, Ying Zhu, Guodong Liu, Fangfu Xu, Qun Hu, Haiyan Wei

PMC · DOI: 10.3390/plants15040668 · 2026-02-23

## TL;DR

This study finds the best nitrogen management for high rice yield and efficient nitrogen use by applying it at specific leaf growth stages.

## Contribution

Identifies optimal nitrogen application rates and timing for maximizing yield and nitrogen efficiency in Nanjing 9108 rice.

## Key findings

- Yield and nitrogen use efficiency increased with nitrogen application up to 270 kg/ha, then decreased.
- Applying nitrogen at the 13th leaf stage improved grain production and nitrogen uptake significantly.
- The N6 treatment increased dry matter accumulation and sustained photosynthesis, leading to higher yields.

## Abstract

The effects of nitrogen (N) application rates and N topdressing at different leaf growth stages on the yield, N absorption, and utilization of japonica rice cultivar Nanjing 9108 were studied to screen the optimal N management mode for high yield and high N use efficiency. A field experiment was conducted from 2023 to 2024, with nine N regulation treatments (94–351 kg ha−1) established through dynamic allocation of basal, tillering, and topdressing fertilizers. The results showed that with the increase of N application rate, the yield and N use efficiency of Nanjing 9108 first increased and then decreased. At a total N application rate of 270 kg ha−1, the N6 treatment (basal N + tiller N + topdressing at the 13th leaf stage) demonstrated optimal overall performance, achieving the highest yield and N use efficiency. Topdressing at the 13th leaf stage (coinciding with young panicle differentiation) promoted spikelet differentiation and large panicle formation, increasing grains per panicle by 2.36–2.20% compared to other treatments under the same N rate. The N6 treatment exhibited enhanced N uptake and utilization: N accumulation increased by 39.27–67.12% during the elongating to heading stage and by 7.14–62.24% during heading to maturity, while N apparent efficiency and agronomic efficiency rose by 3.51–14.68% and 29.22–58.25%, respectively. At heading, the proportion of high-effective leaf area in N6 was 1.52–7.05% higher than in N4, N5, and N7 treatments, accompanied by a slower leaf area decay rate. These traits provided sustained photosynthetic support for dry matter accumulation in mid-to-late growth stages. Consequently, dry matter accumulation in N6 increased by 5.85–33.44% (elongating to heading) and 0.42–26.98% (heading to maturity), leading to a yield advantage of 3.8–17.2% over other treatments. In summary, the N management strategy combining basal, tiller, and 13th-leaf topdressing at 270 kg ha−1 is most effective for achieving both high yield and high N efficiency in Nanjing 9108.

## Linked entities

- **Chemicals:** nitrogen (PubChem CID 947)

## Full-text entities

- **Diseases:** injury to (MESH:D014947)
- **Chemicals:** auxin (MESH:D007210), KCl (MESH:D011189), N2O (MESH:D009609), cytokinin (MESH:D003583), carbohydrates (MESH:D002241), K (MESH:D011188), N4 (-), superphosphate (MESH:C033414), C (MESH:D002244), chlorophyll (MESH:D002734), N (MESH:D009584), P (MESH:D010758)
- **Species:** Oryza sativa (Asian cultivated rice, species) [taxon 4530], Oryza sativa Japonica Group (Japanese rice, no rank) [taxon 39947], Homo sapiens (human, species) [taxon 9606], Oryza sativa Indica Group (Indian rice, no rank) [taxon 39946]

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12944095/full.md

---
Source: https://tomesphere.com/paper/PMC12944095