Employing a Combination of Chemoattractants to Trap Glioblastoma Cells in a Macroporous Hydrogel
Sahar Naasri, Hélène Therriault, Lisa Delattre, Nick Virgilio, Marc-Antoine Lauzon, Nathalie Faucheux, Benoit Paquette
TL;DR
This study explores using a hydrogel trap to attract and retain glioblastoma cells by testing different cytokine combinations to enhance cell migration.
Contribution
The study identifies a novel cytokine combination (IL-1β + CXCL12 + EGF) that effectively attracts diverse glioblastoma cell lines into a hydrogel trap.
Findings
The cytokine combination IL-1β + CXCL12 + EGF was most effective in promoting migration of four GBM cell lines.
U118 cells showed the best accumulation in the macroporous hydrogel when exposed to this cytokine combination.
EGF induced epithelial–mesenchymal transition in all tested GBM cells, facilitating migration.
Abstract
Background: A new paradigm for treating glioblastoma multiforme (GBM) cells was proposed. Instead of trying to eliminate cancer cells infiltrated in the brain, this new treatment is based on attracting them into a macroporous gel-based trap, where they are retained and then irradiated with a localized, higher radiation dose. The objective of this study is to identify a cytokine combination that would attract GBM cells while considering heterogeneity among GBM cell lines. Methods: The ability of different combinations of cytokines CXCL12, IL-1β, IL-6, and EGF to stimulate the migration of the GBM cell lines U87, U87 CXCR4+, F98, and U118 was assessed with a two-layer Matrigel device that simulates the extracellular environment in brain. The accumulation of GBM cells within a cancer cell trap made from a macroporous hydrogel consisting of 1% alginate, 0.75% chitosan, and 0.05% genipin was…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsSaffron Plant Research Studies · Ginger and Zingiberaceae research · Chemokine receptors and signaling