Biological and Biophysical Characterization of Hybrid PLCL Nanofibers Incorporating Stem Cell-Derived Secretome
Tanya Stoyanova, Lora Topalova, Dencho Gugutkov, Regina Komsa-Penkova, Stanimir Kyurkchiev, Iren Bogeva-Tsolova, Dobromir Dimitrov, Svetla Todinova, George Altankov
TL;DR
This study creates hybrid nanofiber scaffolds using PLCL and stem cell secretions to improve tissue regeneration by influencing cell behavior.
Contribution
The novel contribution is the fabrication of aligned and random PLCL nanofibers incorporating stem cell-derived secretome to guide cell behavior.
Findings
Aligned nanofibers led to smaller cell spreading and faster proliferation compared to random fibers.
Aligned nanofibers promoted faster cell migration than random fibers and control substrates.
Abstract
The design of multifunctional biomaterials that offer both structural support and biochemical cues is essential for enhancing tissue regeneration. In this study, hybrid nanofibrous scaffolds composed of poly(L-lactide-co-ε-caprolactone) (PLCL) and bioactive factors secreted by Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) were fabricated via co-electrospinning. Nanofibers were produced in aligned and random configurations following an optimized protocol developed at the Institute for Bioengineering of Catalonia (IBEC). Their morphology and topography were characterized by light microscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM), and fiber orientation was quantified via Fast Fourier Transform (FFT) analysis. The scaffolds showed fiber diameters of 542.9 ± 62.3 nm, with aligned fibers predominantly oriented within 20° of the principal axis. Human…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsElectrospun Nanofibers in Biomedical Applications · Bone Tissue Engineering Materials · biodegradable polymer synthesis and properties