Polymeric PLGA Nanoparticles Loaded with Acalypha monostachya Leaf Hexane Extract: A Novel Strategy for Antineoplastic Activity
Gloria A. Guillén-Meléndez, Carlos R. Montes-de-Oca-Saucedo, Raymundo A. Pérez-Hernández, Priscila Sepúlveda, Flavio F. Contreras-Torres, Rocío Castro-Ríos, Uziel Castillo-Velázquez, María de Jesús Loera-Arias, Humberto Rodríguez-Rocha, Joel H. Elizondo-Luevano
TL;DR
This study shows that encapsulating a plant extract in PLGA nanoparticles improves its cancer-fighting effectiveness in lab tests.
Contribution
Encapsulation of Acalypha monostachya leaf extract in PLGA nanoparticles enhances antineoplastic activity and aqueous dispersibility.
Findings
PLGA nanoparticles with 5/50 extract/polymer ratio had 131.4 nm diameter and 92.03% encapsulation efficiency.
Encapsulated extract showed higher potency with IC50 values of 30 µg/mL (HeLa) and 60 µg/mL (MDA-MB-231).
Selectivity indices of 3.2 and 1.6 were observed for HaCaT/HeLa and HaCaT/MDA-MB-231, respectively.
Abstract
Background/Objectives: Acalypha monostachya is used in rural communities in Mexico as a traditional remedy for cancer, and we previously observed cytotoxic activity of its extracts against MDA-MB-231 and HeLa cells. Methods: Because lipophilic plant fractions disperse poorly in water, we encapsulated the hexane leaf extract (LHE) of A. monostachya in poly (lactic-co-glycolic acid) (PLGA) nanoparticles prepared by nanoprecipitation, characterized them physicochemically, and evaluated their in vitro cytotoxicity. Results: The selected extract/polymer ratio (5/50, w/w) produced nanoparticles with a mean diameter of 131.4 ± 0.5 nm and a PDI of 0.122 ± 0.028, with an encapsulation efficiency of 92.03% and a loading of 8.43%. We next evaluated cytotoxicity by MTT after 24 h in HeLa and MDA-MB-231 cells and compared the response with non-tumorigenic HaCaT keratinocytes. Encapsulation increased…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsPlant-Derived Bioactive Compounds · Pharmacological Effects of Medicinal Plants · Curcumin's Biomedical Applications