A Novel Approach to Biodegradation and Detoxification of Ricinine in Castor Meal: Relationship Between the Gut Microbiota and Microbial Metabolites of Hermetia illucens

TL;DR

This study explores how the larvae of Hermetia illucens tolerate and detoxify ricinine in castor meal through their gut microbiota and microbial metabolites.

Contribution

The study identifies specific gut bacteria and metabolic pathways involved in ricinine degradation and detoxification in Hermetia illucens larvae.

Findings

Ricinine degradation half-life was reduced to 5.13 days in larvae fed with 1000 mg kg−1 ricinine.

Gut bacteria like Dysgonomonas and Actinomyces are linked to ricinine resistance and degradation.

Metabolic pathways related to amino acids and vitamins are enriched in response to ricinine stress.

Abstract

Hermetia illucens can digest toxic castor meal and tolerate ricinine stress. However, the underlying mechanisms of ricinine degradation and detoxification within the larval gut microbiome remain largely unknown. Here, the enhanced degradation kinetic process, and the roles of the gut bacterial community and metabolomics were investigated. When the ricinine content was 1000 mg kg−1 in feeding substrate, larval development was not significantly affected. The ricinine degradation kinetics, facilitated by larval digestion, were significantly enhanced, reducing the degradation half-life to 5.13 days. The gut bacterial community structure adjusted in response to ricinine stress, suggesting that genera such as Dysgonomonas, Actinomyces, Phascolarctobacterium, Lachnoclostridium and Sedimentibacter might play key roles in ricinine resistance and degradation. Furthermore, the gut microbial metabolism responded to toxin stress, reflected by variations in metabolite expression and the enrichment of key metabolic pathways involved in amino acid and vitamin metabolism. This emphasizes the potential role of microbial metabolism in ricinine degradation and detoxification. The close association between gut bacteria and metabolites suggests a cooperative metabolic network within the gut microbiota, where bacteria may participate in ricinine degradation and detoxification either directly or through metabolic cooperation. These findings provide insights into host–microbe interactions and ricinine resistance, highlighting the need for further exploration into the microbiota’s role in host metabolism and the development of new therapeutic strategies.

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