# Valorization of Plant-Based Food By-Products Through Green Extraction of Bioactive Compounds for Functional Food

**Authors:** Cristina-Anca Danciu, Alina-Georgeta Mag, Cristian Stanciu, Livia Vidu, Mirela Stanciu

PMC · DOI: 10.3390/molecules31040646 · Molecules · 2026-02-13

## TL;DR

This review explores how plant-based food by-products can be turned into valuable functional ingredients using eco-friendly extraction methods.

## Contribution

The paper synthesizes biochemical data and processing methods for valorizing by-products into functional food ingredients.

## Key findings

- Fruit and vegetable residues, brewer’s spent grain, and spent coffee grounds contain bioactive compounds suitable for functional food.
- Green extraction techniques improve yield and preserve bioactivity while reducing environmental impact.
- Challenges include standardizing feedstock and validating sustainability through techno-economic assessments.

## Abstract

The revalorization of food processing by-products represents a critical strategy for enhancing resource efficiency and advancing circularity within the food system. This review examines the potential of three major plant-based agro-industrial by-products—fruit and vegetable residues, brewer’s spent grain, and spent coffee grounds—as sources of high-value functional ingredients. These by-products contain bioactive compounds, including dietary fibers, polyphenols, proteins, peptides, oils, and antioxidants, that can be recovered using emerging green extraction and bioprocessing technologies. Conventional extraction methods are progressively being replaced or hybridized with enzyme-assisted, ultrasound-assisted, microwave-assisted, and deep eutectic solvent techniques to improve yield, reduce solvent consumption, and preserve bioactivity. The recovered compounds have demonstrated promising applications as gelling agents (pectin), natural colorants and antioxidants, protein-enriched flours, prebiotic fibers, and bioactive extracts for functional food and nutraceutical formulations. However, challenges persist in standardizing feedstock composition, scaling continuous extraction processes, ensuring safety and regulatory compliance, and generating robust techno-economic and life-cycle assessments to validate sustainability claims. This review synthesizes biochemical composition data, processing pathways, food applications, and regulatory considerations, and identifies research priorities for developing integrated, scalable biorefinery models that valorize food by-products into market-ready functional ingredients.

## Full-text entities

- **Genes:** BSG (basigin (Ok blood group)) [NCBI Gene 682] {aka 5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK}, ALB (albumin) [NCBI Gene 213] {aka FDAHT, HSA, PRO0883, PRO0903, PRO1341}, SI (sucrase-isomaltase) [NCBI Gene 6476], ACE (angiotensin I converting enzyme) [NCBI Gene 1636] {aka ACE1, CD143, DCP, DCP1}
- **Diseases:** cytotoxicity (MESH:D064420), EAE (MESH:D008661), inflammatory (MESH:D007249), injury to (MESH:D014947), cancer (MESH:D009369), diabetic (MESH:D003920)
- **Chemicals:** glucuronic acid (MESH:D020723), betaine (MESH:D001622), caffeine (MESH:D002110), hexane (MESH:D006586), unsaturated fatty acids (MESH:D005231), cafestol (MESH:C053400), Deep Eutectic (-), acarbose (MESH:D020909), lutein (MESH:D014975), carbohydrates (MESH:D002241), fatty acids (MESH:D005227), Oil (MESH:D009821), phenols (MESH:D010636), propionate (MESH:D011422), amino acids (MESH:D000596), urea (MESH:D014508), triethylene glycol (MESH:C028914), arabinogalactan (MESH:C005653), p-coumaric acid (MESH:C495469), TEG (MESH:C000619859), Lipid (MESH:D008055), beta-carotene (MESH:D019207), ABTS (MESH:C002502), FVS (MESH:C536525), phlorizin (MESH:D010695), CO2 (MESH:D002245), Polyphenol (MESH:D059808), BHA (MESH:D002083), lignin (MESH:D008031), Arabinoxylan (MESH:C085118), Heavy metal (MESH:D019216), beta-glucan (MESH:D047071), short-chain fatty acid (MESH:D005232), flavonoid (MESH:D005419), diterpenes (MESH:D004224), chlorogenic acid (MESH:D002726), cellulose (MESH:D002482), hydrogen (MESH:D006859), caffeic acid (MESH:C040048), acetate (MESH:D000085), gallic acid (MESH:D005707), Dietary Fiber (MESH:D004043), sugars (MESH:D000073893), acids (MESH:D000143), metal (MESH:D008670), D-limonene (MESH:D000077222), DPPH (MESH:C004931), Melanoidins (MESH:C011908), choline chloride (MESH:D002794), hesperidin (MESH:D006569), galactomannan (MESH:C012990), coumaric acids (MESH:D003373), Pectin (MESH:D010368), Polysaccharide (MESH:D011134), epicatechin (MESH:D002392), ferulic acid (MESH:C004999), iron (MESH:D007501), phenolic acids (MESH:C017616), kahweol (MESH:C053401), BHT (MESH:D002084)
- **Species:** Daucus carota (carrot, species) [taxon 4039], Listeria monocytogenes (species) [taxon 1639], Powellomyces sp. EA (species) [taxon 252690], Solanum lycopersicum (tomato, species) [taxon 4081], Homo sapiens (human, species) [taxon 9606], Bifidobacterium (genus) [taxon 1678], Bacillus subtilis (species) [taxon 1423], Malus domestica (apple, species) [taxon 3750], Salmonella (genus) [taxon 590], Olea europaea (common olive, species) [taxon 4146], Lactobacillus (genus) [taxon 1578], Theobroma cacao (cacao, species) [taxon 3641]
- **Cell lines:** Caco-2 — Homo sapiens (Human), Colon adenocarcinoma, Cancer cell line (CVCL_0025)

## Full text

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## Figures

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## References

167 references — full list in the complete paper: https://tomesphere.com/paper/PMC12943265/full.md

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Source: https://tomesphere.com/paper/PMC12943265