# Effect of Two Selected Probiotic Leuconostoc mesenteroides Bacteriocin-Producing Strains on Biopreservation and Organic Volatile Compounds in Model Cheese During Ripening and Storage

## TL;DR

This study explores how two probiotic Leuconostoc strains help preserve cheese and improve its aroma during ripening.

## Contribution

The first use of Leuconostoc mesenteroides strains Ln.F5 and Ln.M14 as adjunct cultures in model cheese for biopreservation and VOC enhancement.

## Key findings

- Leuconostoc strains Ln.F5 and Ln.M14 inhibited harmful bacteria like Listeria and E. coli during cheese ripening.
- The strains enhanced cheese quality by producing complex aromatic volatile organic compounds.
- PFGE confirmed the survival of the added Leuconostoc strains in cheese for up to 60 days.

## Abstract

Lactic acid bacteria (LAB) are widely used in food systems; among them, bacteriocin-producing strains have attracted attention for their potential in the biopreservation of dairy products. This study started from the detection of bacteriocin-encoding genes in eight probiotic Leuconostoc mesenteroides subsp. mesenteroides strains, previously isolated, identified, and characterized for antimicrobial activity. Results confirmed the presence of bacteriocin genes across the strains, with Ln.F5 harboring both mesB and lcnA genes, and three other strains, including the Ln.M14 strain, exclusively carrying the lcnA gene. The two strains, Ln.F5 and Ln.M14, were used, in single and mixed cultures, for the first time, as adjunct cultures in a model cheese. Their impact against Listeria spp., Staphylococcus aureus, Escherichia coli, Micrococcus luteus, and Brochothrix thermosphacta, and on volatile organic compounds (VOCs), during ripening and storage, was evaluated. Results showed high viability (9.2 Log CFU/g) of Leuconostoc spp. in model cheese, up to 60 days of storage, and Pulsed-Field Gel Electrophoresis (PFGE) profiles of the re-isolated bacteria confirmed the survival of the added strains. Furthermore, results indicated the inhibition of E. coli and Listeria spp. started from the 15th day of ripening in samples differently inoculated with the two Leuconostoc strains. Listeria spp. was completely inhibited starting from 15 days by Ln.M14, in single culture. The complete inhibition of S. aureus, M. luteus, and B. thermosphacta was detected after 30 days of ripening in samples differently inoculated with Ln.F5 and Ln.M14. The VOC analyses revealed more complex aromatic profiles in samples inoculated with Leuconostoc strains, which, along with the development of cheese eyes, confirmed the effect of the Leuconostoc strains in enhancing quality traits of cheeses.