# Comparing Acute Effects of Caffeine Delivery Forms on Cross-Training Performance: A Randomized Placebo-Controlled Crossover Trial

**Authors:** Salvador Vargas-Molina, Diego A. Bonilla, Manuel García-Sillero, Sergio Iglesias-Placed, Mora Murri, Fernando Martín-Rivera, Javier Benítez-Porres

PMC · DOI: 10.3390/nu18040657 · Nutrients · 2026-02-17

## TL;DR

This study compared how different ways of taking caffeine affect CrossFit performance, finding that caffeine mouth rinse may slightly improve jump performance.

## Contribution

The study evaluates the acute effects of various caffeine delivery methods on CrossFit performance in trained individuals.

## Key findings

- Caffeine mouth rinse significantly improved countermovement jump performance compared to baseline.
- No significant differences were found in internal load measures like RPE, HRmax, or HRmean across caffeine forms.
- Caffeine delivery methods did not significantly affect the total number of repetitions performed.

## Abstract

Background/Objectives: The aim of this study was to compare the different forms of caffeine (CAF) administration in CrossFit® participants. The countermovement jump (CMJ), the rate of perceived exertion (RPE), the total number of repetitions, and the maximum (HRmax) and mean heart rate (HRmean) were evaluated. Methods: Fourteen males with more than six months of continuous CrossFit® training (30.9 [5.62] years, 179 [1.33] cm, 78 [5.75] kg, 24.3 [1.33] kg·m−2) participated in this randomized, placebo-controlled, crossover study. Participants were randomized in a repeated measures design using caffeine capsule (CC), caffeine chewing gum (CCG), and caffeine mouth rinse (CMR) protocols, along with a placebo group (PG). Participants were unaware of whether any of the delivery methods contained caffeine. A 7-day washout period before each crossover was used. To ensure ecological validity, we replicated the real-world practice of CAF ingestion 30 min prior to training, mirroring typical athlete pre-workout routines. The participants of CrossFit® performed the ‘Cindy’ protocol, and the CMJ as a primary outcome was measured pre- and post-intervention, while the RPE, HR, and the number of repetitions were tracked at the end of the workout for comparisons. Results: No significant differences were found between CAF forms in internal load measures (RPE, HRmax, HRmean) or the number of repetitions. While no changes were observed with other CAF forms, CMR significantly improved the CMJ performance compared to the baseline (Δ: +3.5; Cohen’s dunb: 0.51], which exceeded the estimated SWC by approximately three-fold. However, the inferential analysis revealed no significant main effects of the caffeine administration method on any measured outcomes. Conclusions: While CAF delivery forms did not improve internal load measures or performance parameters, such as the RPE, HR, or the total number of repetitions, the caffeine mouth rinse (CMR) showed a potentially meaningful improvement in CMJ performance after CrossFit® training in participants with a certain level of experience.

## Linked entities

- **Chemicals:** caffeine (PubChem CID 2519)

## Full-text entities

- **Genes:** CYP1A2 (cytochrome P450 family 1 subfamily A member 2) [NCBI Gene 1544] {aka CP12, CYPIA2, P3-450, P450(PA)}, MTX1 (metaxin 1) [NCBI Gene 4580] {aka MTX, MTXN}
- **Diseases:** injury (MESH:D014947), CMJ (MESH:C000711648), muscle soreness (MESH:D063806), fatigue (MESH:D005221)
- **Chemicals:** theophylline (MESH:D013806), methylxanthine (MESH:C008514), theobromine (MESH:D013805), carbohydrates (MESH:D002241), CC (-), sodium bicarbonate (MESH:D017693), oxygen (MESH:D010100), CAF (MESH:D002110), maltodextrin (MESH:C008315), norepinephrine (MESH:D009638), water (MESH:D014867)
- **Species:** Homo sapiens (human, species) [taxon 9606], Ilex paraguariensis (mate, species) [taxon 185542]
- **Mutations:** rs762551

## Full text

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## Figures

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## References

44 references — full list in the complete paper: https://tomesphere.com/paper/PMC12942813/full.md

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Source: https://tomesphere.com/paper/PMC12942813