# Commonly Used Analytical Tools and Methods for the Discrimination of Honey Types Based on Volatile Organic Compound Profiles

**Authors:** Gulzhan Khamitova, Simone Angeloni, Lazzat Karasholakova, Giovanni Caprioli

PMC · DOI: 10.3390/molecules31040638 · Molecules · 2026-02-12

## TL;DR

This paper reviews methods for analyzing volatile compounds in honey to determine its origin and quality.

## Contribution

It provides a comprehensive overview of analytical tools and methods for VOC analysis in honey.

## Key findings

- HS-SPME is commonly used for VOC extraction in honey.
- GC-MS and e-nose systems are widely applied for VOC analysis.
- Challenges include variability in VOC profiles and data interpretation.

## Abstract

Honey is a complex natural product with nutritional and therapeutic properties that depend on the diversity of its chemical composition, which includes volatile organic compounds (VOCs). VOCs in honey are key indicators of its botanical and geographical origin, as well as its quality and authenticity. This review provides a comprehensive overview of the analytical instruments and methods used for the identification and quantification of VOCs in different types of honey. Techniques such as headspace solid-phase microextraction (HS-SPME) are used for VOC extraction, and gas chromatography coupled with mass spectrometry (GC-MS) and electronic nose (e-nose) systems for honey analyses, as well as their advantages, limitations, and applications and challenges related to VOC analysis, such as for different types of honeys, their aroma profile, compound variability, and data interpretation, are also discussed. By summarizing recent advancements in analytical methodologies, this review provides an overview of the analysis of VOCs for authentication and research purposes in honey production and processing.

## Full-text entities

- **Genes:** glucose oxidase [NCBI Gene 406081]
- **Diseases:** injury to (MESH:D014947), inflammatory (MESH:D007249)
- **Chemicals:** sulfur (MESH:D013455), terpinolene (MESH:C027009), hydrogen peroxide (MESH:D006861), ethyl acetate (MESH:C007650), 2-ethylhexan-1-ol (MESH:C034017), NaHCO3 (MESH:D017693), CycloSil-B (-), 1-octanol (MESH:D020003), octanoic acid (MESH:C031492), linalool (MESH:C018584), Safranal (MESH:C087963), 2-methoxyphenol (MESH:D006139), amino acid (MESH:D000596), carbohydrate (MESH:D002241), monoterpenes (MESH:D039821), hydrocarbons (MESH:D006838), aliphatic acids (MESH:D005227), carbon dioxide (MESH:D002245), Polydimethylsiloxane (MESH:C013830), fructose (MESH:D005632), HMF (MESH:C008046), Hexanal (MESH:C010463), sesquiterpenes (MESH:D012717), dimethyl trisulfide (MESH:C054170), sucrose (MESH:D013395), lipid (MESH:D008055), alcohols (MESH:D000438), HS (MESH:D006859), furfural (MESH:D005662), hotrienol (MESH:C476493), acetate (MESH:D000085), pentane (MESH:C033353), diethyl ether (MESH:D004986), glucose (MESH:D005947), decanal (MESH:C021170), proline (MESH:D011392), methanol (MESH:D000432), beta-Damascenone (MESH:C075388), azadirachtin (MESH:C010329), ethyl octanoate (MESH:C549324), cineole (MESH:D000077591), succinate (MESH:D019802), Furans (MESH:D005663), uridine (MESH:D014529), sugar (MESH:D000073893), Formic acid (MESH:C030544), methane (MESH:D008697), benzaldehyde (MESH:C032175), dichloromethane (MESH:D008752), Divinylbenzene (MESH:C004985), esters (MESH:D004952), ketones (MESH:D007659), limonene (MESH:D000077222), gluconic acid (MESH:C030691), alpha-pinene (MESH:C005451), 2-butenoic acids (MESH:D003437), 2-phenylethanol (MESH:D010626), nonanal (MESH:C008664), water (MESH:D014867), benzene (MESH:D001554)
- **Species:** Ziziphus jujuba (Chinese jujube, species) [taxon 326968], Litchi chinensis (litchi, species) [taxon 151069], Apis cerana (Asiatic honeybee, species) [taxon 7461], Eucalyptus (genus) [taxon 3932], Homo sapiens (human, species) [taxon 9606], Apis mellifera carnica (Carniolan honeybee, subspecies) [taxon 88217], Apis (genus) [taxon 7459], Gluconobacter (genus) [taxon 441], Trifolium repens (creeping white clover, species) [taxon 3899], Apis mellifera (bee, species) [taxon 7460], Scaptotrigona postica (stingless bee, species) [taxon 79011], Medicago sativa (alfalfa, species) [taxon 3879], Cucumis melo var. inodorus (casaba melon, varietas) [taxon 357961], Helianthus annuus (common sunflower, species) [taxon 4232], Lactobacillus (genus) [taxon 1578], Saccharomyces cerevisiae (baker's yeast, species) [taxon 4932], Apis dorsata (giant honeybee, species) [taxon 7462], Azadirachta indica (Indian-lilac, species) [taxon 124943], Varroa destructor (honeybee ectoparasitic mite, species) [taxon 109461], Tetragonula sp. (species) [taxon 2942419], Zingiber officinale (ginger, species) [taxon 94328]

## Full text

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## References

127 references — full list in the complete paper: https://tomesphere.com/paper/PMC12942777/full.md

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Source: https://tomesphere.com/paper/PMC12942777