# Chemical Composition, Antioxidant and Immunomodulatory Properties of Syrah Grape Seed Extract

## TL;DR

This study shows that Syrah grape seed extract can boost Th1 immune responses in people with lupus, suggesting it may have immunomodulatory benefits.

## Contribution

The study demonstrates the immunomodulatory potential of Syrah grape seed extract in systemic lupus erythematosus, beyond its antioxidant properties.

## Key findings

- GSE increased Th1 cell proportions in both healthy individuals and SLE patients.
- HLA-DR activation marker was upregulated on Th1 cells from SLE patients after GSE treatment.
- No significant effects were observed on Th2 cell populations.

## Abstract

The aim of this study was to obtain and characterize grape seed extract and to demonstrate its potential immunomodulatory properties in the autoimmune disease systemic lupus erythematosus (SLE). A Syrah grape seed extract was obtained using 70% aqueous ethanol, magnetic stirrer, 3 h. The obtained extracts were concentrated by vacuum evaporation and dryer at 40 °C. The total phenolic content (TPC), the total amount of flavonoids (TF) and procyanidins (PC), and the antioxidant (AO) capacity of the extract were determined by spectrophotometric methods. The individual composition of the extract was demonstrated by the high-performance liquid chromatography (HPLC) method. The effect of grape seed extract (GSE) on peripheral blood mononuclear cells (PBMC) from healthy donors and patients with SLE was studied to compare Th1 and Th2 subsets and their expression of key activation markers—CD25 and HLA-DR. PBMC were cultured in the presence or absence of GSE, and the effects on Th1 and Th2 cells were analyzed by flow cytometry. GSE treatment increased the proportion of Th1 cells in both healthy individuals and SLE patients. In addition, a significant upregulation of the late activation marker HLA-DR was observed on Th1 cells obtained from patients with systemic lupus erythematosus (SLE). No significant effects were found on Th2 cell populations. These findings indicate that GSE can stimulate Th1-mediated immune responses in SLE, proving its potential immunomodulatory properties beyond its known antioxidant effects.