Uvpks1, Involved in Ustilaginoidin Biosynthesis, Contributes to the Metabolic Profile, Development, Stress Responses, and Pathogenicity in Villosiclava virens
Xuwen Hou, Jie Dong, Dan Xu, Hao Liu, Yu Li, Yujun Huang, Jiahang Sun, Muhammad Abubakar Jakada, Daowan Lai, Ligang Zhou
TL;DR
This study shows that the Uvpks1 gene in a rice pathogen affects its metabolism, growth, stress tolerance, and ability to cause disease.
Contribution
The study reveals the multifaceted role of Uvpks1 in fungal development and pathogenicity.
Findings
Deleting Uvpks1 stops ustilaginoidin production and activates other metabolic pathways.
Uvpks1 deletion reduces fungal growth, sporulation, and tolerance to certain fungicides.
Uvpks1 influences stress resistance and pathogenicity toward rice plants.
Abstract
Villosiclava virens (anamorph: Ustilaginoidea virens) is a fungal pathogen that causes rice false smut, one of the most devastating diseases of rice. The Uvpks1 gene encoding polyketide synthase is responsible for the biosynthesis of ustilaginoidins, a major group of mycotoxins in V. virens. In this study, three strains, including the Uvpks1 deletion mutant ΔUvpks1, the complementation strain ΔUvpks1-C1, and the wild-type isogenic strain P1 of V. virens, were employed to investigate the role of Uvpks1 in shaping the metabolic profile and in the development, stress responses, and pathogenicity of V. virens. The deletion of Uvpks1 led to both the elimination of ustilaginoidin biosynthesis and the induction of many other secondary metabolite biosynthetic pathways. It decreased mycelial growth and sporulation, fungal tolerance to Congo red-induced cell wall damage stress, and susceptibility…
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Taxonomy
TopicsMycotoxins in Agriculture and Food · Fungal and yeast genetics research · Microbial Natural Products and Biosynthesis