# Analysis of Biological Images and Quantitative Monitoring Using Deep Learning and Computer Vision

**Authors:** Aaron Gálvez-Salido, Francisca Robles, Rodrigo J. Gonçalves, Roberto de la Herrán, Carmelo Ruiz Rejón, Rafael Navajas-Pérez

PMC · DOI: 10.3390/jimaging12020088 · 2026-02-18

## TL;DR

This paper reviews how deep learning and computer vision are used to automate biological counting for wildlife monitoring and biodiversity assessments.

## Contribution

The paper evaluates methodological paradigms and challenges in deep learning for automated biological counting across diverse platforms.

## Key findings

- Deep learning methods achieve over 95% accuracy in counting various taxa using platforms like camera traps and UAVs.
- Challenges include object occlusion, cryptic species differentiation, and lack of high-quality labeled datasets.
- Future improvements require self-supervised learning and better data augmentation for robust ecological monitoring.

## Abstract

Automated biological counting is essential for scaling wildlife monitoring and biodiversity assessments, as manual processing currently limits analytical effort and scalability. This review evaluates the integration of deep learning and computer vision across diverse acquisition platforms, including camera traps, unmanned aerial vehicles (UAVs), and remote sensing. Methodological paradigms ranging from Convolutional Neural Networks (CNNs) and one-stage detectors like You Only Look Once (YOLO) to recent transformer-based architectures and hybrid models are examined. The literature shows that these methods consistently achieve high accuracy—often exceeding 95%—across various taxa, including insect pests, aquatic organisms, terrestrial vegetation, and forest ecosystems. However, persistent challenges such as object occlusion, cryptic species differentiation, and the scarcity of high-quality, labeled datasets continue to hinder fully automated workflows. We conclude that while automated counting has fundamentally increased data throughput, future advancements must focus on enhancing model generalization through self-supervised learning and improved data augmentation techniques. These developments are critical for transitioning from experimental models to robust, operational tools for global ecological monitoring and conservation efforts.

## Full-text entities

- **Diseases:** pine root knot nematodes (MESH:D009349), injury to (MESH:D014947), DL (MESH:C537113), Sunn pests (MESH:D029021), fire (MESH:D000092422), infected (MESH:D007239)
- **Chemicals:** Water (MESH:D014867), Carbon (MESH:D002244), chlorophyll (MESH:D002734), lignin (MESH:D008031), cellulose (MESH:D002482), DNN (-)
- **Species:** Muscidae (house flies, family) [taxon 7366], Picea glauca (white spruce, species) [taxon 3330], Acyrthosiphon pisum (pea aphid, species) [taxon 7029], Fulgoromorpha (planthoppers, infraorder) [taxon 33361], Nicotiana tabacum (American tobacco, species) [taxon 4097], Anopheles (series) [taxon 44484], Cicindelinae (tiger beetles, subfamily) [taxon 27450], Psylloidea (jumping plant lice, superfamily) [taxon 33375], Aedes albopictus (Asian tiger mosquito, species) [taxon 7160], Bactrocera oleae (olive fly, species) [taxon 104688], Pinus contorta (lodgepole pine, species) [taxon 3339], Tephritidae (fruit flies, family) [taxon 7211], Buprestidae (jewel beetles, family) [taxon 50527], Leptinotarsa decemlineata (Colorado potato beetle, species) [taxon 7539], Aphidomorpha (aphids, infraorder) [taxon 33380], Sorghum bicolor (broomcorn, species) [taxon 4558], Lepidoptera (moths & butterflies, order) [taxon 7088], Apis mellifera (bee, species) [taxon 7460], Pinus subgen. Pinus (diploxylon pines, subgenus) [taxon 139271], Drosophila melanogaster (fruit fly, species) [taxon 7227], Coleoptera (beetles, order) [taxon 7041], Diptera (flies, order) [taxon 7147], Apis cerana (Asiatic honeybee, species) [taxon 7461], Formicidae (ants, family) [taxon 36668], Cistus ladanifer (species) [taxon 335173], Aedes (subgenus) [taxon 149531], Catostomidae (suckers, family) [taxon 7968], Solanum lycopersicum (tomato, species) [taxon 4081], Populus tremuloides (quaking aspen, species) [taxon 3693], Homo sapiens (human, species) [taxon 9606], Hymenoptera (hymenopterans, order) [taxon 7399], Vespidae (wasps, family) [taxon 7438], Rhynchophorus ferrugineus (Asian palm weevil, species) [taxon 354439], Bursaphelenchus xylophilus (pine wilt nematode, species) [taxon 6326], Culex (subgenus) [taxon 53527]

## Figures

3 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12941886/full.md

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Source: https://tomesphere.com/paper/PMC12941886