Regulation of ABC Transporters and Ergosterol Biosynthesis by the Transcription Factor FvADS-1 Controls Azole Resistance and Virulence in Fusarium verticillioides
Yajing Yin, Hanxing Zhang, Zhenying Zhang, Mi Zhou, Shaojie Li, Chengcheng Hu
TL;DR
A transcription factor called FvADS-1 helps Fusarium verticillioides resist antifungal drugs and be more harmful to plants.
Contribution
FvADS-1 is shown to regulate azole resistance and virulence through ABC transporters and ergosterol biosynthesis.
Findings
FvADS-1 deletion increases susceptibility to azole compounds.
FvADS-1 regulates genes involved in sterol homeostasis and oxidative stress tolerance.
FvADS-1 contributes to virulence on maize plants.
Abstract
Fusarium verticillioides is a significant agricultural pathogen and an emerging causative agent of invasive fusariosis in clinical settings. Fusarium species frequently exhibit resistance to available antifungal agents, yet the molecular mechanisms underlying azole resistance remain poorly characterized. In this study, we identified the Zn(II)2Cys6 transcription factor FvADS-1 as a positive regulator of the azole stress response in F. verticillioides. The transcription of FvADS-1 was significantly induced by ketoconazole (KTC), and its deletion increased susceptibility to multiple azole compounds. Mechanistically, FvADS-1 positively regulates the KTC-induced expression of genes encoding ABC transporters and ergosterol biosynthesis enzymes, thereby modulating intracellular KTC accumulation and sterol homeostasis under azole stress. Furthermore, FvADS-1 positively regulates the…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsFungal and yeast genetics research · Antifungal resistance and susceptibility · Mycotoxins in Agriculture and Food