Brevetoxin Metabolites: Emerging Toxins in French Shellfish Determined by LC-MS/MS and ELISA
Zouher Amzil, Amélie Derrien, Korian Lhaute, Aouregan Terre Terrillon, Simon Tanniou
TL;DR
French researchers detected brevetoxin metabolites in shellfish using LC-MS/MS and ELISA, finding high concentrations that exceed safety guidelines.
Contribution
A two-step monitoring strategy combining ELISA and LC-MS/MS is proposed to accurately assess brevetoxin contamination in shellfish.
Findings
Four brevetoxin metabolites (BTX-2, BTX-3, BTX-B5, S-deoxy-BTX-B2) were quantified using LC-MS/MS.
ELISA detected brevetoxins in all Corsican shellfish samples, with higher concentrations than LC-MS/MS.
Brevetoxin levels exceeded French safety guidelines, prompting a new monitoring strategy.
Abstract
In France, as part of the monitoring program for the emergence of marine toxins in shellfish (EMERGTOX), brevetoxins (BTX-2, BTX-3) were first detected in shellfish from Corsica (Mediterranean Sea) in 2018. The complex metabolic transformation of brevetoxins in shellfish, coupled with the limited availability of analytical standards for most metabolites, complicates the accurate evaluation of contamination levels. To address this challenge, two complementary analytical approaches were implemented to quantify brevetoxin metabolites in shellfish samples collected from 2018 to 2023: (i) a targeted LC-MS/MS method specially developed for brevetoxins; and (ii) an ELISA capable of detecting metabolites for which no reference standards are available. Of the 11 brevetoxin metabolites targeted, 4 were quantified by LC-MS/MS: BTX-2, BTX-3, BTX-B5, and S-deoxy-BTX-B2 (including its isomers). The…
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Taxonomy
TopicsMarine Toxins and Detection Methods · Water Treatment and Disinfection · Mycotoxins in Agriculture and Food