# Myosin Class I Genes Follow a Cell Type-Specific Transcription Pattern in Human Haematopoietic Cell Lines

## TL;DR

This paper studies how different myosin class I genes are expressed in human blood cell lines, revealing cell-specific patterns that could help understand their roles in disease.

## Contribution

The study introduces a qPCR system and re-analyzes RNA-seq data to reveal cell-type-specific expression patterns of all eight human MYO1 genes.

## Key findings

- MYO1 genes show restricted and cell-type-specific expression patterns in human cell lines.
- A qPCR system was developed and validated for screening human myosin I expression.
- The study highlights unique expression profiles in haematopoietic cell lines.

## Abstract

Class I myosins form an abundant group of single-headed motor proteins that interact with actin and membrane phospholipids and are employed in a variety of cell processes like cell shape remodeling, motility and invasion, exocytosis, and endocytosis. The large number of class members and their uneven distribution in human cells and tissues complicate their study. Furthermore, this hinders the assessment of their biomarker potential and mechanistic role in oncogenesis and other diseases. Despite the emerging data on their mutations and altered abundance in a number of tumors and other diseases, most studies examine class I myosins individually or in groups of several proteins. Thus, the question of their redundancy and possible functional substitution is ignored. In this work, we simultaneously assess all eight human MYO1 genes on cell lines of different origins by RNA-seq re-analysis. We also report a qPCR system for human myosin I screening and test it on a set of 35 continuous cell lines widely used in biomedicine and fundamental research. These data clarify the restricted expression patterns of MYO1 genes in various human cell types and allow to assess the pattern specific of haematopoietic cell lines.