# Chitosan-Loaded Inorganic Oxide Nanocomposites (SiO2, ZnO, CuO) for Effective Control of Postharvest Fungal Diseases and Maintaining Apple Fruit Quality

## TL;DR

Scientists created chitosan-based nanocomposites that effectively fight fungal diseases in apples, offering a sustainable alternative to traditional fungicides.

## Contribution

The study introduces scalable, eco-friendly chitosan-inorganic oxide nanocomposites with dual antifungal and host-defense-enhancing properties.

## Key findings

- CS-CuO nanocomposites showed over 80% growth inhibition against Botrytis cinerea and Alternaria alternata.
- In vivo trials on apples demonstrated an 81% reduction in lesion area with CS-CuO treatment.
- CS-CuO treatment increased total phenolic content and antioxidant activity in infected apples.

## Abstract

Phytopathogenic fungi pose a critical threat to global food security through substantial pre- and post-harvest crop losses, intensified by climate change and fungicide resistance. To address this, we synthesized low-concentration chitosan–inorganic oxide nanocomposites (CS-SiO2, CS-ZnO, CS-CuO) via ionic gelation, a green and scalable method. Comprehensive characterization (DLS, UV-Vis, FTIR, XRD, SEM) confirmed nanocomposite formation, CS-SiO2 exhibited uniform particle sizes (200–250 nm), while CS-CuO showed slightly larger particles, all with excellent dispersity. Zeta potential analysis confirmed strong colloidal stability, with pure chitosan nanoparticles (CSNPs) displaying a surface charge of +12.9 mV, while all nanocomposites retained positive charges, enhancing adhesion to negatively charged fungal membranes. In vitro antifungal assays against Alternaria alternata, Botrytis cinerea, Colletotrichum graminicola, and Fusarium graminearum demonstrated hierarchical efficacy: CS-CuO > CS-ZnO > CS-SiO2, with CS-CuO achieving >80% growth inhibition against B. cinerea and A. alternata. SEM revealed severe hyphal damage and spore collapse in CS-CuO-treated fungi, attributed to synergistic reactive oxygen species (ROS) generation and chitosan-mediated membrane disruption. In vivo trials on B. cinerea-infected apples showed CS-CuO reduced lesion area by 81% and elevated host defense markers, including a 1.5-fold increase in total phenolic content and higher DPPH radical scavenging activity compared to controls. These nanocomposites, particularly CS-CuO, offer a sustainable, dual-action solution direct antifungal activity and enhanced host resilience while minimizing environmental impact. By integrating scalable synthesis, eco-compatibility, and efficacy, this work advances chitosan–inorganic oxide nanocomposites as viable alternatives to conventional fungicides, with immediate potential for agricultural and postharvest applications.